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Analysis of gene expression in maternal and fetal tissues exposed to cigarette smoke
Fejglová, Kamila ; Bruchová, Hana (advisor) ; Zikánová, Blanka (referee)
Despite widespread awareness of the adverse health effects of smoking, pregnant women expose themselves to this factor (firsthand and/or secondhand smoke). Cigarette smoke exposure during pregnancy increases the risk of preterm delivery, abortion or newborn sudden death syndrom. To study molecular mechanisms of cigarette smoke exposure in tissues of pregnant women and their newborns, we employed genomic approach and assessed gene expression profiles in leukocytes from maternal peripheral blood and umbilical cord blood and in placenta as well. Gene expression profiles of these tissues were determined by Illumina HumRef8 v3 expression chips that enable analysis of 23 811 coding, annotated transcripts. Microarray data were normalized using the quantile method. Differential gene expression was analyzed using moderated t-statistic. A linear model was fitted for each gene given a series of arrays using lmFit function. Multiple testing correction was performed using the Benjamini and Hochberg method. Comparative analysis of gene expression profiles between smokers (N=23) and non-smokers (N=62) revealed many deregulated genes, demonstrating a negative effect of cigarette smoke on transcriptome of the tested tissues. In peripheral blood of smokers, we detected overexpression of these genes; e.g. IFI27, IFIT1L,...
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Analysis of gene expression in maternal and fetal tissues exposed to cigarette smoke
Fejglová, Kamila ; Zikánová, Blanka (referee) ; Bruchová, Hana (advisor)
Despite widespread awareness of the adverse health effects of smoking, pregnant women expose themselves to this factor (firsthand and/or secondhand smoke). Cigarette smoke exposure during pregnancy increases the risk of preterm delivery, abortion or newborn sudden death syndrom. To study molecular mechanisms of cigarette smoke exposure in tissues of pregnant women and their newborns, we employed genomic approach and assessed gene expression profiles in leukocytes from maternal peripheral blood and umbilical cord blood and in placenta as well. Gene expression profiles of these tissues were determined by Illumina HumRef8 v3 expression chips that enable analysis of 23 811 coding, annotated transcripts. Microarray data were normalized using the quantile method. Differential gene expression was analyzed using moderated t-statistic. A linear model was fitted for each gene given a series of arrays using lmFit function. Multiple testing correction was performed using the Benjamini and Hochberg method. Comparative analysis of gene expression profiles between smokers (N=23) and non-smokers (N=62) revealed many deregulated genes, demonstrating a negative effect of cigarette smoke on transcriptome of the tested tissues. In peripheral blood of smokers, we detected overexpression of these genes; e.g. IFI27, IFIT1L,...
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