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Application of voltammetry for simultaneous determination of selected microelements
Bruštík, David ; Komendová, Renata (referee) ; Řezáčová, Veronika (advisor)
This bachelor thesis concentrates on the optimization of conditions for voltammetric determination of selected microelements, specifically zinc, copper, cadmium and lead. The theoretical part describes the individual properties of selected elements and their toxicity, occurrence and important figures of concentration. The practicable methods suitable for the determination of these microelements are summarized and the voltammetric analysis is described in depth. The experimental section deals with the optimization of dissolution voltammetry conditions, the description of the method used and the analysis of selected real samples.
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Characterization of Rhodosporidium toruloides proteome using LC-MS/MS
Bruštík, David ; Szotkowski, Martin (referee) ; Zdráhal, Zbyněk (advisor)
Characterization of differentially regulated proteins is crucial for the identification of metabolic pathways and their understanding in connection with the creation of important products of a selected strain of yeast. This diploma thesis focuses on the proteome analysis of the Rhodosporidium toruloides cultivated under different conditions. The metabolism of these yeasts with the characteristics of important metabolites is described in the theoretical part. The next part of the thesis is focused on proteomic approaches and bottom-up proteomics from the sample preparation to mass spectrometry analysis. The experimental part deals with the cultivation of yeast at different C/N ratios, next the isolation and determination of proteins using the FASP method, which includes proteolytic cleavage by trypsin, LC-MS/MS analysis and the database search.
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Characterization of Rhodosporidium toruloides proteome using LC-MS/MS
Bruštík, David ; Szotkowski, Martin (referee) ; Zdráhal, Zbyněk (advisor)
Characterization of differentially regulated proteins is crucial for the identification of metabolic pathways and their understanding in connection with the creation of important products of a selected strain of yeast. This diploma thesis focuses on the proteome analysis of the Rhodosporidium toruloides cultivated under different conditions. The metabolism of these yeasts with the characteristics of important metabolites is described in the theoretical part. The next part of the thesis is focused on proteomic approaches and bottom-up proteomics from the sample preparation to mass spectrometry analysis. The experimental part deals with the cultivation of yeast at different C/N ratios, next the isolation and determination of proteins using the FASP method, which includes proteolytic cleavage by trypsin, LC-MS/MS analysis and the database search.
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Application of voltammetry for simultaneous determination of selected microelements
Bruštík, David ; Komendová, Renata (referee) ; Řezáčová, Veronika (advisor)
This bachelor thesis concentrates on the optimization of conditions for voltammetric determination of selected microelements, specifically zinc, copper, cadmium and lead. The theoretical part describes the individual properties of selected elements and their toxicity, occurrence and important figures of concentration. The practicable methods suitable for the determination of these microelements are summarized and the voltammetric analysis is described in depth. The experimental section deals with the optimization of dissolution voltammetry conditions, the description of the method used and the analysis of selected real samples.
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