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Immunohistochemical detection of PECAM-1, endoglin and VCAM-1 in aorta of transgenic mice with high levels of soluble endoglin
Brlicová, Monika ; Rathouská, Jana (advisor) ; Filipský, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Immunohistochemical detection of PECAM-1, endoglin and VCAM-1 in aorta of transgenic mice with high levels of soluble endoglin Rigorous thesis Mgr. Monika Brlicová Consultant of rigorous thesis: PharmDr. Jana Rathouská, Ph.D. Background: We observed an effect of high level of human soluble endoglin (sEng) on an expression of tissue endoglin and adhesion molecules VCAM-1 and PECAM-1 in descendent aortas of experimental animals. Methods: 6 months old female transgenic mice of strain CBAxC5B7BL/6J with high plasma levels of human soluble endoglin (Sol-Eng+ ) and the same types of mice, which show signs of low plasma levels of human soluble endoglin as control mice were used for this rigorous thesis. Both groups were fed by high fat diet for 3 months. After that the levels of total cholesterol in the blood of these animals were biochemically determined and then statistically evaluated. The immunohistochemical method ImmPRESSTM was used for detection of protein expression in aortic sections. Results: Biochemical analysis showed a statistically insignificant difference in total cholesterol levels in both groups. Immunohistochemical analysis ImmPRESSTM showed no differences in the expression of...
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Evaluation of endoglin and P-selectin expression and co-expression in aortas of apoE-deficient mice
Brlicová, Monika ; Rathouská, Jana (advisor) ; Kovařík, Miroslav (referee)
Charles University in Prague Faculty of Pharmacy in HradecKrlov Department of Biological and Medical Sciences Evaluation of endoglin and P-selectin expression and co-expression in aortas of apoE-deficient mice Diploma thesis MonikaBrlicov Supervisor: Mgr.JanaRathousk Background: We observed the expression and the reciprocal co-expression of endoglin (receptor III for TGF- cytokine) and P-selectin (adhesion molecule and marker of endothelial dysfunction) in ascending aortas of apoE-deficient mice which were fed by standard diet for rodents and Western type diet (high-cholesterol diet) for achieving of different phases of the atherosclerotic process. The changes of total cholesterol levels in mice after administration of both types of diets were also evaluated. Methods: The modified strain C57BL/6J of mice with a deficiency of apolipoprotein E, which is prone to aterogenesis was used for this diploma thesis. Mice were divided into three groups. The first group was fed by standard diet (so-called "chow" diet) for a period of two months and the second two groups were fed by Western type diet for a period of two and four months. The levels of total cholesterol in the blood were biochemically determinated and then we statistically evaluated this levels in all groups. Immunohistochemical...
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Immunohistochemical detection of PECAM-1, endoglin and VCAM-1 in aorta of transgenic mice with high levels of soluble endoglin
Brlicová, Monika ; Rathouská, Jana (advisor) ; Filipský, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Immunohistochemical detection of PECAM-1, endoglin and VCAM-1 in aorta of transgenic mice with high levels of soluble endoglin Rigorous thesis Mgr. Monika Brlicová Consultant of rigorous thesis: PharmDr. Jana Rathouská, Ph.D. Background: We observed an effect of high level of human soluble endoglin (sEng) on an expression of tissue endoglin and adhesion molecules VCAM-1 and PECAM-1 in descendent aortas of experimental animals. Methods: 6 months old female transgenic mice of strain CBAxC5B7BL/6J with high plasma levels of human soluble endoglin (Sol-Eng+ ) and the same types of mice, which show signs of low plasma levels of human soluble endoglin as control mice were used for this rigorous thesis. Both groups were fed by high fat diet for 3 months. After that the levels of total cholesterol in the blood of these animals were biochemically determined and then statistically evaluated. The immunohistochemical method ImmPRESSTM was used for detection of protein expression in aortic sections. Results: Biochemical analysis showed a statistically insignificant difference in total cholesterol levels in both groups. Immunohistochemical analysis ImmPRESSTM showed no differences in the expression of...
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Evaluation of endoglin and P-selectin expression and co-expression in aortas of apoE-deficient mice
Brlicová, Monika ; Rathouská, Jana (advisor) ; Kovařík, Miroslav (referee)
Charles University in Prague Faculty of Pharmacy in HradecKrlov Department of Biological and Medical Sciences Evaluation of endoglin and P-selectin expression and co-expression in aortas of apoE-deficient mice Diploma thesis MonikaBrlicov Supervisor: Mgr.JanaRathousk Background: We observed the expression and the reciprocal co-expression of endoglin (receptor III for TGF- cytokine) and P-selectin (adhesion molecule and marker of endothelial dysfunction) in ascending aortas of apoE-deficient mice which were fed by standard diet for rodents and Western type diet (high-cholesterol diet) for achieving of different phases of the atherosclerotic process. The changes of total cholesterol levels in mice after administration of both types of diets were also evaluated. Methods: The modified strain C57BL/6J of mice with a deficiency of apolipoprotein E, which is prone to aterogenesis was used for this diploma thesis. Mice were divided into three groups. The first group was fed by standard diet (so-called "chow" diet) for a period of two months and the second two groups were fed by Western type diet for a period of two and four months. The levels of total cholesterol in the blood were biochemically determinated and then we statistically evaluated this levels in all groups. Immunohistochemical...
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