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The Proposal of Business Plan for the Establishment of a Bicycle Shop
Kobližková, Markéta ; Bártová, Eva (referee) ; Mucha, Martin (advisor)
This bachelor´s thesis deals with creating (floatating) entrepreurial plan for a fictive company in connection with requirements and attributes of founding and driving. First part contains theoretical knowledge of aspects of founding and driving the firm, structure and terms of a entrepreurial plan. In the next part theory is put into practice. Practical part is already the entrepreurial plan for establishing of a company offering products and services in the area of cycling plant in the Czech Republic.
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Abilities and importance long-term cultivation embryonic cells from different species of animals
Houdek, Zbyněk ; Vožeh, František (advisor) ; Bártová, Eva (referee) ; Pokorný, Jaroslav (referee)
Embryonic cells inside the blastocyst, which divide and differentiate into cells of all tissues of organism, are called embryonic stem (ES) cells. Using the ES cells for transplantations is one the potential ways in regenerative medicine. The present study deals with two different lines of mouse pluripotent cells: embryonic carcinoma (EC) cells line P19 and ES cells line D3. The in vitro cultivation was performed in basal cultivation medium with fetal bovine serum (FBS) and in addition of leukemia inhibitory factor (LIF) in case of ES cells. The neurodifferentiation was induced by medium without FBS and without LIF in case of ES cells and supported by retinoic acid in case of P19 cells and embryoid bodies cultivation in case of ES cells. Pluripotency and neurodifferentiation of the cells were confirmed by presence of molecular markers of pluripotency and neurodifferentiation in both lines in nondifferentiated and differentiated states. Mouse EC P19 cells were transfected by gene for green fluorescent protein (GFP) and used for transplantation in cerebellum of wild type (WT) and Lurcher (Lc) mutant mice. Nondifferentiated cells (P19) and neuroprogenitors (NPG) derived from these cells were used. The study followed the survival, morphology and localization of the grafts. The survival of both types of GFP...
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Financování vysokých škol v ČR a v Austrálii
Bártová, Eva
The aim of this bachelor thesis is to propose recommendations for financing universities in the Czech Republic with the possibility of using private sources of students. The first part of the thesis describes the basic types of universities financing systems in the world. The system of financing universities in the Czech Republic and Australia is analysed in detail so as loans for college students in Australia. Based on the results of the EUROSTUDENT projects and the questionnaire for students of the Faculty of Business and Economics of the Mendel University in Brno, financial resources and economic activity of students are compared. Part of the thesis is an analysis of financing systems in the Czech Republic and Australia using data from OECD Education at and Glance reports. The obtained outputs are discussed and possible utilization of the principles of Australian system of financing of universities in the Czech Republic is assessed.
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A Microfluidic devide for monitoring cell migration
Ledvina, Vojtěch ; Klepárník, Karel ; Legartová, Soňa ; Bártová, Eva
A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes chemotactic movement of cells in the direction of increasing gradient of fetal bovine serum. HeLa cell line transfected with Premo FUCCI Cell Cycle Sensor was used as a model organism. The cells were imaged for several days using a scanning confocal microscope and the rate of migration in different cell cycle phases was evaluated.
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Regulation of the DNA damage response by R2TP mediated MRN complex assembly and control of 53BP1 localisation.
Von Morgen, Patrick ; Hořejší, Zuzana (advisor) ; Bártová, Eva (referee) ; Kleibl, Zdeněk (referee)
DNA double strand breaks are the most dangerous type of DNA damage. The MRN complex and 53BP1 have essential functions in the repair of DNA double strand breaks and are therefore important for maintaining genomic stability and preventing cancer. DNA double strand breaks are repaired by two main mechanisms - homologous recombination and non- homologous end joining. The MRN complex senses DNA double strand breaks and activates a cascade of posttranslational modifications that activates and recruits other effector proteins. In addition MRN mediated resection is important for removing adducts in non-homologous end joining and creating single stranded DNA required for homologous recombination. 53BP1 is recruited to DNA double strand breaks by site specific ubiquitinations and inhibits DNA resection, thereby promoting non-homologous end joining at the expense of homologous recombination. In this thesis we show that MRE11 binds to the R2TP chaperone complex through a CK2 mediated phosphorylation. Knockdown of R2TP or mutating the MRE11 binding site leads to decreased MRE11 levels and impaired DNA repair. Similar phenotype has been observed in cells from patients with ataxia-telangiectasia-like disorder (ATLD), containing MRE11 deletion mutation which is missing the R2TP complex binding site. Based on R2TP...
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Abilities and importance long-term cultivation embryonic cells from different species of animals
Houdek, Zbyněk ; Vožeh, František (advisor) ; Bártová, Eva (referee) ; Pokorný, Jaroslav (referee)
Embryonic cells inside the blastocyst, which divide and differentiate into cells of all tissues of organism, are called embryonic stem (ES) cells. Using the ES cells for transplantations is one the potential ways in regenerative medicine. The present study deals with two different lines of mouse pluripotent cells: embryonic carcinoma (EC) cells line P19 and ES cells line D3. The in vitro cultivation was performed in basal cultivation medium with fetal bovine serum (FBS) and in addition of leukemia inhibitory factor (LIF) in case of ES cells. The neurodifferentiation was induced by medium without FBS and without LIF in case of ES cells and supported by retinoic acid in case of P19 cells and embryoid bodies cultivation in case of ES cells. Pluripotency and neurodifferentiation of the cells were confirmed by presence of molecular markers of pluripotency and neurodifferentiation in both lines in nondifferentiated and differentiated states. Mouse EC P19 cells were transfected by gene for green fluorescent protein (GFP) and used for transplantation in cerebellum of wild type (WT) and Lurcher (Lc) mutant mice. Nondifferentiated cells (P19) and neuroprogenitors (NPG) derived from these cells were used. The study followed the survival, morphology and localization of the grafts. The survival of both types of GFP...
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The Proposal of Business Plan for the Establishment of a Bicycle Shop
Kobližková, Markéta ; Bártová, Eva (referee) ; Mucha, Martin (advisor)
This bachelor´s thesis deals with creating (floatating) entrepreurial plan for a fictive company in connection with requirements and attributes of founding and driving. First part contains theoretical knowledge of aspects of founding and driving the firm, structure and terms of a entrepreurial plan. In the next part theory is put into practice. Practical part is already the entrepreurial plan for establishing of a company offering products and services in the area of cycling plant in the Czech Republic.
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Cattle Breeding under Eco-agricultural Conditions
BÁRTOVÁ, Eva
The diploma thesis is divided into three parts: cattle herd management, ethology and economic evaluation. The observation was carried out on the farm which is situated in the mountain region of Šumava mountain with decreasing agricultural production. The main production is cattle breeding without market milk production. These meat breeds (Hereford, Aberdeen angus, Charolais) are bred there.
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Possibility of DNA probes preparation for want of CMG
Krejčí, Jana ; Bártová, Eva
The main method of cytogenetics is the DNA fluorescence in situ hybridization (DNA-FISH), where DNA probe links to the specific locus of genome. In our laboratory, DNA probes are prepared from DNA amplified by bacterial vector (PAC/BAC). The following step is exploiting Nick-translation reaction for incorporation of reporter molecule targeted for antibody conjugate with fluorescence molecule. Specificity of prepared DNA probe is increased using Cot-DNA, which suppress the repetitive sequences.
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