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Effects Evaluation of Quinazolin Alkaloids from Justicia Adhatoda
Žampachová, Pavla ; Vopršalová, Marie (advisor) ; Karlíčková, Jana (referee)
v anglickém jazyce Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Mgr. Pavla Žampachová Supervisor: PharmDr. Marie Vopršalová, CSc. Title of rigorous thesis: Effects evaluation of quinazolin alkaloids from Justicia adhatoda The aim of our experiment was the evaluation of a relaxing activity of natural substances obtained from Justicia adhatoda by an in vitro method on an isolated trachea of rats. Specifically, we tested: a standardized ethanol extract from Justicia adhatoda, vasicine and vasicinone. As a standard substance, we used theofylline. Theofylline was also used for inducing maximal relaxation of a trachea. Our experiment was carried out on rat males of Wistar tribe, weight between 250-300 g. The scheme of our experiment was in following sequence: inducing trachea contractions by giving a carbachol solution (10-5 M), a cumulative intake of separate doses of a tested substance ensued; finally, theofylline (3×10-3 M) was used for inducing maximal trachea relaxation. Every measured value of relaxation of tested substances was compared to maximal relaxation by theofylline and issued with percentage. Acquired values were compiled by GraphPad Prism 5 program. Through this program, for every tested substance, we gained a DRC...
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Effects Evaluation of Quinazolin Alkaloids from Justicia Adhatoda
Žampachová, Pavla ; Vopršalová, Marie (advisor) ; Karlíčková, Jana (referee)
v anglickém jazyce Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Mgr. Pavla Žampachová Supervisor: PharmDr. Marie Vopršalová, CSc. Title of rigorous thesis: Effects evaluation of quinazolin alkaloids from Justicia adhatoda The aim of our experiment was the evaluation of a relaxing activity of natural substances obtained from Justicia adhatoda by an in vitro method on an isolated trachea of rats. Specifically, we tested: a standardized ethanol extract from Justicia adhatoda, vasicine and vasicinone. As a standard substance, we used theofylline. Theofylline was also used for inducing maximal relaxation of a trachea. Our experiment was carried out on rat males of Wistar tribe, weight between 250-300 g. The scheme of our experiment was in following sequence: inducing trachea contractions by giving a carbachol solution (10-5 M), a cumulative intake of separate doses of a tested substance ensued; finally, theofylline (3×10-3 M) was used for inducing maximal trachea relaxation. Every measured value of relaxation of tested substances was compared to maximal relaxation by theofylline and issued with percentage. Acquired values were compiled by GraphPad Prism 5 program. Through this program, for every tested substance, we gained a DRC...
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