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National Repository of Grey Literature

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Advanced methods of assaying lectin-carbohydrate interaction Červený, Jakub ; Bosáková, Zuzana (advisor) ; Pavlíček, Jiří (referee) A reliable and reproducible quantification and detailed characterization of the interactions between a biomedically relevant protein and its synthetic carbohydrate ligand can provide new information for the design of better diagnostic and therapeutic tools. A high sensitivity and specificity are essential requirements for the feasibility of an analytical method. These parameters can be difficult to achieve for highly complex systems such as lectin-saccharide complexes. On the other hand, carbohydrate-based diagnostics and therapeutics offer the great advantage of detecting biologically active lectin receptors, which cannot be achieved with antibodies. In this work we demonstrate the use of the BLI technique to monitor lectin- carbohydrate interactions for a multivalent system. Using functionalization of a novel construct of galectin-1 with an in vivo biotinylated AVI tag, suitable immobilization on a biosensor was achieved without the loss of its lectin activity. Due to the high sensitivity of this technique, kinetic parameters of interaction with prepared multivalent neoglycoproteins based on LacNAc epitopes were obtained in the micro- to nanolar KD range. The complementarity of this method was demonstrated by parallel ITC and competitive ELISA measurements. The neoglycoprotein with a lower LacNAc... Detailed record

Enzymatic synthesis and analysis of bioactive oligosaccharides Červený, Jakub ; Bojarová, Pavla (advisor) ; Kavan, Daniel (referee) Detailed record

See also: similar author names
7	ČERVENÝ, Jan
8	ČERVENÝ, Jiří
7	Červený, Jan
11	Červený, Jaroslav
8	Červený, Jiří

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