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Blood-testis barrier - role in the regulation of spermatogenesis in pathological conditions.
Častulová, Kristýna ; Semecký, Vladimír (advisor) ; Herink, Josef (referee)
Background The aim of this thesis was to bring the theoretical part of the latest findings on the structural composition and functionality of the blood-testis barrier and its importance in working with biological agents that would influence the development and maturation of gametes. In the experimental part, the possible impairment of the complexity of the blood-testis barrier by application of model xenobiotics - busulfan - was studied by histopathological analysis. Methods Adult male Wistar rats, which were treated with busulfan intaperitoneally, were used for the experiment. The first group of animals received 1 dose of busulfan (40 mg / kg body weight). The second group of rats were given two doses of busulfan (10 mg / kg body weight) 21 days apart (days 1 and 21). Animals were divided into 6 groups and sacrificed 7, 14, 21, 28, 42 and 70 days after the second busulfan dose. Immediately after sacrificing rats, the abdominal cavity was opened and the testicles removed. After removal of adipose tissue, the length and weight of both testes were recorded. The testicles were then subjected to histological processing. The prepared histological preparations were evaluated under a light microscope. Results The archival histological material of the Department of Biological and Medical Sciences was used...
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