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How to prevent the proliferation of Legionella pneumophylis in hot water in the hospital Czech Krumlov
TUREK, Jan
This diploma thesis deals with microbiological quality of hot water in a hospital in Cesky Krumlov as well as hot water piping condition with respect to Legionella. It also deals with arrangements for improving the quality of hot water in this hospital. The subject and target of this thesis are the findings of the mentioned parameters based on own observations, technical documentation and analysis of warm water performed by an accredited laboratory. The hospital in Cesky Krumlov, the construction of which began in 1909, was built in 1911 as the 'Nemocnice Císaře Františka Josefa I.' As the time went by, new buildings were built up. The hospital was renamed to 'Všeobecná veřejná nemocnice' in 1942. In the following years the hospital expanded until the current condition. Water quality and presence of Legionella is a world-wide issue, which we must fight against. The thesis is divided into two parts. First, theoretical part, which includes both general and specific knowledge about Legionella, its health risks, the environment in which it occurs, possibilities of eliminating, suitability of the material for hot water pipes, gained in professional literature and a part of the Czech legislation dealing with public health protection, the law 'No. 258/2004 Coll.' in actual version and quality of drinking water and hot water. In the second, empirical part, I focus on the situation of the hospital in Cesky Krumlov from 2009 till 2015, concerning newly built boiler room and the whole distribution system of hot water regarding Legionella. Particularly I focused on materials used for ecologisation of the gas boiler room and materials used for hot water pipes and actual state of water distribution in individual buildings of this health care facility. I mentioned the analysis of the water samples performed by an accredited laboratory with corresponding technical documentation, which concerns the water distribution system, provided by the medical facility. I used secondary data analysis to evaluate these documents. Regarding the results from 2009 to 2015 the microbiological quality of warm water doesn't meet the limits defined in the Attachment No. 3 of the above mentioned law Order 252/2004 Coll. Limit value for colony forming units of Legionella is set to maximum of 100 CFU. In most of the samples this limit was exceeded by series of units up to hundred thousands of units. The last results in 2015 prove improvement of the unfavorable situation. In that year the analysis was tested three times. First in February, where the CFU units were 'only' in hundreds, with the top value of 550 CFU in the Postacute Care ward. Second analysis was performed in June that same year. Results were acceptable. The highest value was 64 CFU. Last analysis in the hospital was performed in December. Increased concentration of Legionella was again in the Surgery building. There were established of 1400 colony forming units. There were 300 CFU in the Postacute Care ward and the limit value of 100 CFU was in the ward of Internal medicine. The answers to research questions 'How to prevent reproduction of Legionella peumophylis in the water distribution system of the hospital in Cesky Krumlov?' and 'How to ensure a sufficient amount of quality hot water in the medical facility?' are included at the end of the stated recommendations. A risk factor causing the fast reproduction of Legionella is the old water distribution system in most of the buildings and low water temperature of water leaving the boiler, which is 55°C. All mentioned results and evaluations are included in this thesis as well as attached pictures, which show the environment and situation within the Health Care Institutions.
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Účinky xenobiotik na oxidační stres, metabolizmus lipidů, integritu DNA a životaschopnost lidských buněk a rybích spermií in vitro
LINHARTOVÁ, Pavla
Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing a serious threat to the survival of aquatic organisms including fish. In addition balancing risks and benefits of fish consumption is nowadays an intensively discussed public health topic. Spermatozoa of almost all fish species are released into water environment where they can be directly exposed to various compounds, such as xenobiotics including toxic metals, prior to fertilization. In addition, exposure of parental adults to various xenobiotics may affect gamete quality, which may subsequently reduce fertilization success. On the other hand the advantages of eating fish are well-known, not only in the point that fish is a healthy source of protein and other nutrients, but eating contaminated fish may also confer various health benefits. Research over the past few decades has shown that the nutrients and particularly the n-3 fatty acids (FA) found in fish and seafood, are for examples protective against cardiac diseases and have a positive impact on brain development. The thesis provides a focus on two different cell model types. Firstly, human hepatocellular cells (Hep G2, ATCC) were used as in vitro tool for studying the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, oxidative stress and fatty acid and phospholipid class compositions. Secondly, spermatozoa of one threatened species of fish, sterlet (Acipenser ruthenus) were used as in vitro model for studying effect of potentially hazardous xenobiotic compounds' occurring in open waters. Sperm from sterlet were exposed for 2h to environmentally relevant concentrations of DQ (0-150
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Nové postupy biomonitoringu cizorodých látek ve vodním prostředí
ČERVENÝ, Daniel
Within the presented work, several approaches of biomonitoring were studied. A well-established method for human health risk assessment was applied to evaluate the quality of fish from open waters in the Czech Republic. As this work is targeted on sport fishermen, who frequently consume their own catches, besides the publication in scientific journal, a brochure with results of this study was distributed via the Czech Fishing Union. Health risks for consumers related to the consumption of wild fish were found strongly species and locality dependent. As there is no risks related with the consumption of carp (Cyprinus carpio) at all investigated sites, frequent consumption of predatory fish should be avoided at some fishing grounds. The use of passive samplers has become more and more attractive in the last two decades. As these devices are able to mimic the biological uptake of chemicals, their potential for replacing fish as bioindicators in routine biomonitoring programmes is evident. In our study, the comparison between analysis of fish tissues and POCIS extracts from selected sampling sites confirms this idea about interchangeability of these indicators in the case of PFASs as target pollutants. Moreover, no metabolic transformation of contaminants present in water take place in passive samplers contrary to living organisms, thus these reflect the actual contamination at the locality more precisely. The use of passive samplers completely fulfils the internationally accepted principles of Replacement, Reduction, Refinement (the three R´s). Another promising approach using YOY fish as bioindicators was evaluated within the present study. Multispecies samples of YOY fish homogenate was found as a better indicator for wide range of pollutants than muscle tissue of adult fish within one species from the same locality. As all inner organs and tissues are presented in analysed samples, more target pollutants in higher concentrations are detected compared to the muscle of adults. Besides the higher sensitivity in pollutants detection and quantification, practical and economic benefits are of concern in the case of the YOY fish approach. The YOY fish approach also particularly fulfil the three R´s strategy, as sampling of this age group of fish has a smaller impact on the environment than the sampling of adults. In case of mercury, that was found as a limiting contaminant for the consumption of fish from open waters in the Czech Republic, a nonlethal method of finclips sampling can be used for the monitoring of this compound in aquatic environments. Besides the monitoring, our optimised method for the estimation of muscle tissue concentrations could be used for human health risk assessment as well. The difference between real muscle concentrations and concentrations estimated using our method differs less than 10% in most of the investigated localities. Benthic organisms are an important part of the food web in aquatic environments, but insufficient information about its contamination is available. In another study included in this thesis, first evidence about bioaccumulation of certain pharmaceuticals in benthic organisms was described. Although, pharmaceuticals are generally considered to be not accumulative in organisms, our study proved that certain pharmaceuticals have bioaccumulation potential. From this finding, it is evident that not only the concentrations of pharmaceutical dissolved in water but also the diet represents an important exposure pathway of contamination by these compounds for organisms (fish) inhabiting aquatic environments.
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The effect of pyrethroid based pesticides on fish
RICHTEROVÁ, Zuzana
Pyrethroids are ones of the most used pesticides worldwides. The widespread use and high stability of pyrethroids lead to the assumption of that their occurrence in the environment could be quite frequent. They can reach water ecosystem as pollutants. Residues of pyrethroids are not only detected in the water column, but also in sediments and in fish tissues. The first study was devoted to the product Nexide containing 60 g.l-1 of active substance gamma-cyhalothrin. Tested Nexide concentrations were 5, 25, 50, 100, and 250 µg.l-1. Early life stage test was used.Common carp (Cyprinus carpio) was tested. There were significant mortalities in all concentrations except the lowest concentration during the trial. The lowest concentration tested 5 µg.l-1 only caused a slightly increased mortality. This lowest concentration influenced the growth in length and weight negatively, decelerated ontogenetic development, and made the body surface of the individuals darker. Histopathology of individuals from this concentration revealed dystrophy in liver. Examination of kidney, intestine and gills did not show significant histopathological differences compared with control. The evaluation of selected parameters of oxidative stress demonstrated a significantly higher activity of detoxification enzyme glutathione-S.transferase (GST) and a significantly lower activity of defensive enzyme glutathione peroxidase (GPx) compared with the control group. The other examined parameters of oxidative stress such as catalase (CAT), glutation reductase (GR), and lipid peroxidation determined by using the thiobarbituric acid-reactive substances (TBARs) were comparable to the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Nexide in the given concentration leads to dysbalance of defensive enzymes. The second study was devoted to the product Cyperkill 25 EC containing 250 g.l-1. Tested Cyperkill 25 EC concentrations were 7.2, 36, 72, 144, and 360 µg.l-1. The procedure of the trial was the same as the preceded one. There were 100% mortalities in all concentrations except the lowest concentration during the trial. The lowest tested concentration 7.2 µg.l-1 allowed 90% of individuals to stay alive till the end of experiment. The lowest concentration influenced the growth in length and weight negatively and decelerated ontogenetic development compared with the control. Any individual exposed to this concentration did not reach juvenile stage until the end of the trial. Dark pigmentation was visible in 68% of these exposed individuals on the last day. Similar darkening was visible in individuals from higher concentrations shortly before death too. Histological examination did not revealed significant changes in intestine, liver, kidney, and gills compared with the control group. Evaluation of selected parameters of oxidative stress demonstrated significantly lower activities of GST, GR, and GPx. Activities of CAT and TBARS were comparable with the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Cyperkill 25 EC in the given concentration could induce oxidative stress and interfere with the activities of antioxidant enzymes. The presented thesis summarises actual data about pyrethroids and their influence on fish. The demonstrated effects confirm high susceptibility of early developmental stages of fish to tested pesticides. When interpreting the results, we have to take into account the fact that studies showed this risk even on single pyrethroid substances. But water organisms are exposed to many other more or less toxic products and substances in a real environment. These xenobiotics could react with each other and their mixture could even potentiate negative effects. The performed studies also clearly show the significant differences in the sensitivity of embryonic and embryolarval tests.
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Security and stability networking testing in cloud computing
Efimov, Igor ; Havránek, Martin (advisor) ; Ladislav, Ladislav (referee)
The purpose of this dissertation work involves creation and automation of my own cloud security and stability testing techniques.
The basis of this project is to explore and outline the virtual environment protection level in front of different types of security and stability networking attacks.
Theoretical part of this paper consists of concepts and, together with their definitions and reference to relevant research literature, science related publications and online sources that best describes testing methodology of cloud computing, focused on security and virtualization.
The practical part of the thesis demonstrate an understanding of theories and concepts that been studied. Based on the obtained research results, have been developed various
automation tests that are going to be run on virtual environment in order to test their stability.
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Occurrence of PBDE compounds and Bisphenol A in rivers with the occurrence of cryfish
Svobodová, Kateřina ; Vach, Marek (advisor)
The thesis deals with result evaluation of a project under the auspices of the EAA and Norway Grants, which ran from 2015 to 2016. The project's goal was to evaluate water quality at sites with native and invasive crayfish. The project also included monitoring organic substances such as Polybrominated diphenyl ethers (PBDEs). I mainly focused on a subset of PBDEs, the brominated flame retardants (BFRs). These substances have been monitored since the 1970s, when food was accidentally contaminated leading to the contamination of the nearby area in the US. What is alarming is their persistence in the environment, which leads to their constantly increasing levels. Information about the transformation and biodegradation of PBDEs in the environment is still limited and is continually analyzed in new studies. These substances are poorly volatile and insoluble, which makes dust and particles arising from the products containing BPAs the main sources of contact. Another agent, whose presence in the environment of the Czech Republic I analyzed, is Bisphenol A (BPA). BPA is used in the production of epoxy resins and polycarbonate plastics. BPA is a toxic substance with the ability to disrupt hormone function. Baby feeding bottles have been confirmed to contain these compounds. In April 2004, the Czech government approved a (program to reduce pollution of surface waters by dangerous harmful substances). In August 2004, the EU enacted a ban on the sale and use of penta-BDE and octa-BDE in all fields. Since 2011, Bisphenol A may not be used in the manufacture of baby feeding bottles. The goal of this thesis is to analyze and evaluate the amount and possible consequences of these substances in our environment, which are alarming even after full or partial bans on the use of these substances.
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Freshwater mussels breeding methods for the purpose of conservation programs
Plechingerová, Věra ; Douda, Karel (advisor) ; Matasová, Klára (referee)
Bachelor thesis is written in an overview form of problems breeding freshwater molluscs. At the beginning of the thesis I first evaluate the overall status of the populations in the Czech Republic and then in the world. Next I describe the anatomy and biology of molluscs, especially their reproduction, which is crucial for rearing juveniles in the laboratory or seminatural environment. The return of molluscs populations into the waters ecosystems is problematic in terms of ongoing changes in the biotope, particularly because of the anthropogenic influences and too few suitable host fish, without which it can not complete its glochidia development. To prevent the complete extinction of some species, there have been researched different methods of rearing, which help recover viable populations in the natural water environment. Methods of rearing are also described in this thesis. In the experimental part of the thesis I evaluate types of detritus that have different effects in length gain of mussels. For the experiment was selected freshwater pearl mussel (Margaritifera margaritifera), whose population is highly endangered. Freshwater mussels are an important bio-indicator of waters ecosystems, where they have an irreplaceable function, so it is important to regenerate their populations and the overall condition of the biotopes.
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Historical development of stands in the first zone of the National Park Šumava close to the village Prášily
Strnad, Jiří ; Šálek, Lubomír (advisor) ; Zahradník, Daniel (referee)
The dissertation, The History of Evolution of the Forests of the Category I Protected Area in the National Park Šumava- environs of the municipality of Prášily, firstly focuses on the research and analysis of extant historical documents and other archival materials. In particular it analyses the historical maps of the former Velkostatek Prášily-Dlouhá Ves of the Schwarzenbergs family that show the evolution of the forests and their management between 1860 and 1930.
Secondly, the dissertation focuses on the assessment of the current state of the forests and the way in which they are managed by the Správa NP Šumava and in particular on the positioning of the category I protected areas in order to protect the remnants of the native woodland.
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Constitutional rights for a favorable environment and it´s enforcement in modern Czech Republic
Tauchmanová, Renata ; Kadlecová, Eva (advisor) ; Uhlík, Milan (referee)
The thesis provides the most comprehensive summary of the legal protection
of the environment in the Czech Republic from the perspective of the justice and it is application in the Czech judiciary. Especially in regards to laws on the protection
of the right to a favourable environment within the Constitution of the Czech Republic,
I have tried to concentrate on the jurisprudence of the Constitutional Court, as to the court, whose decisions are very crucial for the interpretation and further application of the law
to the favourable environment in the practice.
The goal of my thesis is to show the evolution of the legal / legislative environment
in the Czech Republic and to approach the practice of the international law, especially european law in the field of the environmental protection. The thesis outlines available trial impalements, which uses the czech judiciary in the field of the law for a favourable envi-ronment and also represents the rights and obligations of the czech citizens related to this topic.
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Waste prevention and effective waste management in the selected company
Míšková, Markéta ; Mikulová, Vlastimila (advisor) ; Slavomír, Slavomír (referee)
From reason of protection environment, under the pressure of not only administrative, but economic measures too are firms trying to minimise the amount of nascent waste. Therefor is thesis focused to the analysis of waste economy, production and composition of waste in the company WITTE Nejdek in period since 2010 to 2015. There is evaluated dependence the amount of waste similar to communal on number of employees in the company WITTE Nejdek. Was done landscaping survey, what was focused to possibilities of reducing amount of wastes, to quality of recycling waste and to availability of collecting points in the company areal. To evalaution of current dondition of recycling wastes in the company WITTE Nejdek was done survey with employees, what was focused on awareness and knowledge employees about recycling waste in WITTE Nejdek. In these are proposed concrete measures, what could improve recycling of waste and make changes helping to reduce amount of waste. Proposals are suitable not only in terms of prevention to waste production, but economically too.
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