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Design and construction of Src biosensor
Pavlík, Vojtěch ; Rösel, Daniel (advisor) ; Brdička, Tomáš (referee)
Src kinase is a well-known proto-oncogene that contributes to cell migration and proliferation and is often found deregulated in tumors. Yet, biology of Src is not fully understood and great effort is made to find new tools for broadening knowledge about the kinase. In this thesis is described design and construction of a novel Src biosensor that exploits genetically encodable fluorophores derived from green fluorescent protein and Förster/fluorescence resonance energy transfer (FRET). The fluorophores are inserted directly into the structure of full-length c-Src in the way that should not impair the inner regulatory mechanisms of Src. The created biosensor proved to be sensitive to various stimuli, which also activate c-Src, by increase of activating autophosphorylation on Tyr4162 and decrease in FRET. Preliminary experiments indicate that the Src biosensor can be used to reflect Src activation in fixed cell as well.

MRI Data Processing Acceleration on GPU
Kešner, Filip ; Nečas, Ondřej (referee) ; Polok, Lukáš (advisor)
This BSc Thesis was performed during a study stay at the Universita della Svizzera italiana, Swiss. The identification of trajectories of neuron fibres within the human brain is of great importance in many medical applications as the neural diagnostics, neuronavigation, treatment of epilepsy, surgical removal of tumors and etc. By using diffusion MRI-data as input, and by employing Monte-Carlo like methods, possible trajectories are generated and the most likely ones can be visualized. These can serve as input for advanced medical diagnosis and treatments. Due to the huge amount of data to be analyzed and many iterations, this is a time consuming process. For the purposes such as statistical analysis and comparsion over several datasets or several patients, computational time requirements are enourmous. Faster diagnosis can improve routine throughput and provide earlier treatment of illness. At this time, there exists only a very few implementations of neural tractography sof tware. For probabilistic neural tractography is the list of software even thiner. Today's implementations using standard serial CPU execution suffer from high time consumption. The goal is to provide an efficient implementation which makes use of GPGPUs and exploits parallelism in the method. For the GPU implementation, a comparsion of CUDA and OpenCL technologies will be provided, using the more suitable one.