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Activity of novel acridine dyes - in vitro study
Pavlová, Dominika ; Macháček, Miloslav (advisor) ; Novotná, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Dominika Pavlová Supervisor: RNDr. Miloslav Macháček, PhD. Title of diploma thesis: Activity of novel acridine dyes - in vitro study Acridine derivatives are being investigated for their potential in the treatment of many diseases, such as cancer, bacterial infections, and malaria. For example, amsakrin, acting as a topoisomerase poison that interacts with both DNA and human topoisomerase II, is used in clinical practice in conventional antitumor chemotherapy. Derivatives of 9-aminoacridine-4- carboxidoamide act similarly. The master's thesis investigates the in vitro activity of the newly synthesized 9-aminoacridine-4-carboxidoamide derivatives and other derivatives of acridine. The purpose of the thesis was to determine whether the studied compounds FK-8-HCl, FK-20-HCl, FK-27-HCl, FK-36-HCl, FK-42-HCl, FK-46-HCl, FK-48, FK-78-HCl, FK-97- HCl and FK-106 are cytotoxic, what concentration induce inhibition of proliferation, what type of cell death is triggered and whether they inhibit the human topoisomerase II. The human epithelial cell line HeLa was used for cytotoxicity experiments. The neutral red uptake assay was used to determine the cell viability. Cells were exposed to different...
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