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Effect of tapeworm (Hymenolepis diminuta) on bioaccumulation of zinc in the host (Rattus norvegicus)
Sloup, Vladislav ; Jankovská, Ivana (advisor)
This research investigates the ability of rat tapeworm (Hymenolepis diminuta) to accumulate zinc in tissue and the influence of its intake and excretion by its host (laboratory rat). The host was fed by food with zinc in two forms: 1) a mixture of standard food ST1 with hyperaccumulator plant Arabidopsis halleri, 2) ST1 mixture with zinc lactate, which is ordinarily used as a feed supplement for increasing zinc content and it is often included in human diet supplements. Rat control group fed by ST1 only was included in the experiment for verification of the difference. Rats were divided to six groups (OO, OT, RT, RO, MO a MT). Three rat groups were infected by rat tapeworm (OT, RT, MT) and three rat groups were not infected (OO, RO, MO). The control groups OO and OT were fed by ST1 only. RO and RT groups were fed by ST1 with admixture of Arabidopsis halleri and groups MO and MT were fed by ST1 with zinc lactate. Urine and excrements of the rats were collected twice a week during the experiment and their amounts were measured every day. The rats were weighted every week. At the end of the balance phase of the experiment rats were euthanized and seven selected tissues were removed (liver, kidney, spleen, small intestine, testis, muscle and bone). Rat tapeworm was removed from the infected rats. Blood was drawn from the rats. The results show that rat groups infected by the rat tapeworm had lower concentration of zinc in almost all analyzed tissues except for spleen, where the concentration of zinc was the same as in groups without the rat tapeworm. Based on the results the rat tapeworm also has an influence on the excretion of feces and urine.
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The use of bioimpedance analysis for kidney function evaluation
Veselková, Alena ; Šrámek, Petr (advisor) ; Švára, František (referee)
Objective: To calculate glomerular filtration (GF) through the use six available methods to estimate GF and measure impedance parameters phase angle () and fat free mass (FFM) by using multifrequency and monofrequency measurement. Select one method of estimating GF as a strict method of estimation GF for this study and by using linear regression relate with other method of estimating GF and impedance parameters measured () and FFM. Consider if () and FFM are significant parameters to improve methods of estimating GF given below (MDRD-A, MDRD-B, CLCG, CLcysC). (...) Conclusions: Impedance parameters () and FFM correlate with GF, decreasing GF cause decreasing of these impedance parameters. This study proved very close relationship between parameters FFMx and FFMc, obtained by using two different kinds of measuring (R2 - FFMx and FFMc = 0,843). Method of bioimpedance is ready to other validation and to develope bioimpedance scale to estimate renal function.
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Strategies for determinig transfer prices in period of economical crisis
Skrbková, Lucie ; Francírek, František (advisor) ; Jakoubek, Jiří (referee)
In the most cases the market range of transfer pricing is determined from statistical data from previous years. In case of these data the time delay occurs when these data are collected and these data do not reflect if that are collected for period of economical growth or recession. As a result, the final transfer pricing range can become distorted. For this reason it is essential to be familiar with strategies and procedures that can be applied to "clean up" statistical data from the influence of business cycle fluctuations. These strategies and procedures are discussed in this diploma thesis. In the first theoretical chapter are presented transfer pricing issues, set the basic terms, analyzed basic available resources dealing with transfer pricing, defined methods for determining transfer pricing range and identified consequences of the economic crisis in relation to transfer pricing. The second chapter is focused on the selection of the most appropriate method for determining transfer pricing and comparative of both the theoretical and practical basis. The last chapter presents the strategies and procedures that can be used in a comparative analysis, in order to eliminate distortions of the statistical data from the influence of business cycle fluctuations.
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Participative management – key to minimaze conflicts between protection of biodiversity and socio-economic development of local communities
Těšitel, Jan ; Kušová, Drahomíra ; Matějka, K. ; Boháč, J. ; Bartoš, Michael ; Kopáčková, M. ; Moravcová, Jana ; Šrubař, Vladan
The project was aimed to propose a model of optimal coexistence of biodiversity protection and socio-economic development in large-scale protected areas. Its main output was identification of principles of participative management of protected areas and estimation of circumstances necessary for its practical implementation. The project focused on description of biodiversity in particular model areas, on eventuality of changes in biodiversity due to human activities and thus on identification of actual and potential conflicts. In parallel, selected parameters of quality of life of local population, behaviour the population and its relation to the Administration of protected area and to nature protection in general were studied. Analysis of behaviour of the administration of protected areas as social institution became also part of the project. The project was designed as empirical comparative study of three biosphere reserves (BR) - BR Šumava, BR Třeboňsko a BR Křivoklátsko.
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Evaluation of the effectiveness of prevention of tobacco smoking in secondary schools in the district Strakonice
HOCHOVÁ, Tereza
This thesis deals with the issue of smoking. It is a serious global problem. Illnesses and death associated with smoking are among the most important problems of Public health in most countries. The risk of damage threatens not only to active smokers but also those who are exposed to passive smoking. Abuse of tobacco is clearly linked to cardiovascular diseases, cancer and other serious diseases. Most smokers begin to smoke no longer in secondary schools. According to Kalina (2003) is modus 14 years. Therefore, preventive activities must be realized before this period. School (outside of family and peers) affects education, attitudes and values of the student. Compulsory school attendance does from primary schools ideal environment for implementation preventive activities. That is reason why I wanted to know if and how well schools use an education as a means of primary prevention. Theoretical part contains the terminology associated with tobacco issues, further a history of tobacco and methods of tobacco and nicotine use. The following chapters provide information of substances contained in cigarettes, selected diseases, prevention, legislation, cessation and withdrawal symptoms and methods of quitting. I used a quantitative method for research in the practical part. Respondents were selected from among pupils from primary schools of Strakonice. At each of the schools in Strakonice I addressed to 50 pupils. First half of them were educated and second half were not. Two hundred of students were interviewed together. I discarded two questionnaires. Total quantity of respondents was 198 students. Questionnaire contained 20 questions, which was designed for students of second grade of elementary school. I set three targets. The main target was to map the problem of smoking among pupils of primary schools in Strakonice, evaluate the impact of education on the knowledge of the respondents and ascertain the views of students on the quality of education. Research has shown that the prevalence of smokers among students of second grade of elementary school in Strakonice is lower than prevalence by GYTS. Older pupils (who were educated) smoke to the greater extent (15.3 %) than younger (uneducated) pupils (6 % smokers). More than 70 % of smokers want to quit the smoking. Almost 26 % of respondents tolerate smoking of other people. Impact of education was assessed by the knowledge part of the questionnaire. It contained eight questions with a choice of four answers, only one was correct. The quality of information obtained in the school, most pupils assessed as good or marginal. These three hypotheses were created: the prevalence of smokers among respondents is higher with higher age, there is a statistically significant difference in knowledge of the issue of smoking between educated and uneducated pupils, and school provides respondents more information than other sources. For statistical verification was used the chi-square test at a significance level of 5 %. The first and second hypothesis was confirmed. A third hypothesis was refuted. Most pupils answered: media (television, internet...), school and family. The quantity of answers was balanced and so we can't talk about a statistically significant difference. Survey has enabled me to become a teacher for two hours. I was able teach sixth grade pupils in the issue of smoking. I have gotten so complex idea about the role of education in primary prevention. I was also convinced that it is necessary to grasp the issue from the perspective of pupil, not teacher. The theoretical part of the thesis can serve as educational material for students, teachers and the public. The practical part can be used to compare the results with other works or to monitor trends of smoking in Strakonice.
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Audit of risk control in quality management
Maslíková, Dominika ; Nosek, Aleš (referee) ; Fiala, Alois (advisor)
The master´s thesis analyses the current state of risk management of the production process. The theoretical part describes the risk management process, selected tools and methodologies for the identification, analysis and assessment. The practical part of the thesis provides a methodology for auditing risk management. One of the goals is to verify applicability of the audit in manufacturing organization. Practical understanding of the risk management process and identification of potential operational risks in the risk areas are analysed using FMEA method.
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The influence of selected ECJ judgements on the forming of common commercial policy of EU
Vicherek, Ondřej ; Bič, Josef (advisor) ; Procházková Ilinitchi, Cristina (referee)
The goal of my diploma thesis is to describe the influence of ECJ decision making on the common commercial policy ("CCP") of EU. Specifically my thesis examines what factors influence ECJ decision making about direct effect of trade agreements implementing CCP. These factors are applied on several treaties, where the court has not yet decided on their potential direct effect and on a present case at ECJ on direct effect of WTO treaties. With regard to the goal of my thesis an important part of my thesis deals with the development of ECJ decision making. Methods applied in my thesis are analysis and comparison of selected judgements that influence the CCP of EU. After proper analysis I found out that the main motiv behind ECJ decision making is that, if the negotiating position of EU is to be deteriorated, the ECJ is likely to find that the treaty does not have direct effect. Other factors are of secondary character. Examining the influence of decision making of ECJ on direct effect of trade agreements is important, because the role of ECJ influences the legal certainty of subjects about direct applicability of international trade treaties.
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The chosen validation parameters of method for assessment of aroma compounds in elderberries
Christovová, Silvia ; Ürgeová, Eva (referee) ; Vítová, Eva (advisor)
This bachelor thesis deals with the assessment of aroma active compounds in elderberries (Sambucus nigra L.). In the theoretical part the chemical composition and attributes of elderberries are described. Brief information on their healing properties is also given. The possible processing methods in food industry, as well as the traditional means of use at home, are discussed furthermore. Following chapters describe the gas chromatography method combined with the technique of solid-phase microextraction, utilized for the specification of aroma active compounds. The validation of this method is also included. The aim of the experimental part of the work was to verify a set of the selected validation parameters of the SPME-GC method, specifically linearity, the limits of detection (LOD), the limits of quantification (LOQ) and repeatability. Subsequently, the SPME-GC method was applied on the chosen elderberry juice samples. In total, 37 different volatile aroma active compounds were identified and quantified, 15 of which belonged to alcohols, 9 to aldehydes, 5 to ketones, 5 to esters and 2 other compounds. The chemical composition of individual samples was not uniform; no single sample contained all of the identified compounds.
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Potenciální antimikrobiální účinky vybraných přírodních látek
MAŠKOVÁ, Hana
This study was focused on potential antiviral and antibacterial effects of viniferin and some derivatives of adamantane. The most suitable concentration was determined using flow cytometer. The antiviral effect on tick-borne encephalitis virus infection in glioblastoma cells was determined. Virus concentration was determined using plaque assay. The antibacterial effect was detected by the incubation of bacterial strains with suitable concentration of selected natural substances. The rate of bacterial growth was determined using absorbance measurement at 600 nm and plating on agar plates.
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