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The Controlling Study
Herda, Tomáš ; Mikovcová, Hana (advisor) ; Herda, Zdeněk (referee)
The main goal of this Diploma´s Thesis is to make a model for calculation of water and sewer rates for the company Vodovody a kanalizace Náchod, a.s. when sticking to the set criteria both from the side of VaK Náchod, a.s. and law regulations. Based on the theoretical part an analysis of customer sensitivity to the price changes using the data for last 20 years follows. Findings from the first two parts are used in risk analysis in next part. The created model calculates the water and sewer rates based on the information from the company accounting system in the way to generate sufficient financial resources to fulfill the renovation plan of infrastructural property plant and equipment and to transfer given amount to the company funds. In addition, the model monitors whether the legal condition of maximal allowable increase of profit per m3 is met. In the customer sensitivity to the price changes part the price elasticity of demand for water and sewer rates is calculated based on the data from 1995 to 2015. The assumption of inelastic demand is confirmed. Risk analysis part is deals with potential risk regarding the demand and prices. Potential impacts for the most significant risk are quantified. The analysis uses knowledge gained in the first two parts. It was confirmed that potential risks are exiting but do not have any significant impact on the going concern of VaK Náchod, a.s. The created model has been already used for the calculation of prices for the year 2017. Customer sensitivity analysis to the price changes and link to the potential risks is an additional information for VaK Náchod, a.s. which validates that nowadays, there are no significant threats which could affect the demand and water and sewer rates significantly.
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Modelling, parameter estimation, optimisation and control of transport and reaction processes in bioreactors.
ŠTUMBAUER, Václav
With the significant potential of microalgae as a major biofuel source of the future, a considerable scientific attention is attracted towards the field of biotechnology and bioprocess engineering. Nevertheless the current photobioreactor (PBR) design methods are still too empirical. With this work I would like to promote the idea of designing a production system, such as a PBR, completely \emph{in silico}, thus allowing for the in silico optimization and optimal control determination. The thesis deals with the PBR modeling and simulation. It addresses two crucial issues in the current state-of-the-art PBR modeling. The first issue relevant to the deficiency of the currently available models - the incorrect or insufficient treatment of either the transport process modeling, the reaction modeling or the coupling between these two models. A correct treatment of both the transport and the reaction phenomena is proposed in the thesis - in the form of a unified modeling framework consisting of three interconnected parts - (i) the state system, (ii) the fluid-dynamic model and (iii) optimal control determination. The proposed model structure allows prediction of the PBR performance with respect to the modelled PBR size, geometry, operating conditions or a particular microalgae strain. The proposed unified modeling approach is applied to the case of the Couette-Taylor photobioreactor (CTBR) where it is used for the optimal control solution. The PBR represents a complex multiscale problem and especially in the case of the production scale systems, the associated computational costs are paramount. This is the second crucial issue addressed in the thesis. With respect to the computational complexity, the fluid dynamics simulation is the most costly part of the PBR simulation. To model the fluid flow with the classical CFD (Computational Fluid Dynamics) methods inside a production scale PBR leads to an enormous grid size. This usually requires a parallel implementation of the solver but in the parallelization of the classical methods lies another relevant issue - that of the amount of data the individual nodes must interchange with each other. The thesis addresses the performance relevant issues by proposing and evaluation alternative approaches to the fluid flow simulation. These approaches are more suitable to the parallel implementation than the classical methods because of their rather local character in comparison to the classical methods - namely the Lattice Boltzmann Method (LBM) for fluid flow, which is the primary focus of the thesis in this regard and alternatively also the discrete random walk based method (DRW). As the outcome of the thesis I have developed and validated a new Lagrangian general modeling approach to the transport and reaction processes in PBR - a framework based on the Lattice Boltzmann method (LBM) and the model of the Photosynthetic Factory (PSF) that models correctly the transport and reaction processes and their coupling. Further I have implemented a software prototype based on the proposed modeling approach and validated this prototype on the case of the Coutte-Taylor PBR. I have also demonstrated that the modeling approach has a significant potential from the computational costs point of view by implementing and validating the software prototype on the parallel architecture of CUDA (Compute Unified Device Architecture). The current parallel implementation is approximately 20 times faster than the unparallized one and decreases thus significantly the iteration cycle of the PBR design process.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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CD46 and β1integrin interaction in mouse sperm head
Šebková, Nataša ; Frolíková, Michaela ; Dvořáková-Hortová, Kateřina
CD46 protein plays an important role during fertilization and its role is associated with acrosome stability. CD46 is probably involved in signalling pathways triggering the acrosome reaction. Integrins interact with many cytoskeletal proteins such as actin, therefore changes in the actin cytoskeleton before and after AR may lead to changes in the association and localization of CD46 and β1integrin. Our aim was to monitor mutual CD46 and β1integrin interaction detected by the proximity ligation assay. It generates a localized signal in a form of spots revealing the exact position of the recognition event. Proteins interaction was study in freshly released sperm and sperm during the acrosome reaction, during which there is a gradual relocation of these proteins towards the equatorial segment and the whole sperm head. Proteins α and β tubulin were used as a positive control, α tubulin and β1 integrin as a negative control. In situ PLA showed a distinct spotted signal indicating the mutual interaction of CD46 and β1integrin. A positive response was demonstrated not only in freshly released sperm but also in sperm during the acrosome reaction. Freshly released sperm were distinctively labelled in the acrosome region and the neck, similarly to the positive control. Sperm during the acrosome reaction showed the signal across the whole sperm head region. No signal or sporadic nonspecific staining was detected in the case of the negative control. In summary, our results deliver new information that proteins CD46 and β1 integrin interact with each other. These results suppose the theory that β1 integrin can mediate a connection between CD46 and sperm cytoskeleton thereby molecules of signalling pathways leading to activation of the acrosome reaction.
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The comparison of growth performance of beef breed Aberdeen Angus twins
KOCÁBEK, Jan
Aberdeen Angus (originated in Scotland) is the second most common beef breed in the Czech Republic due to its resistance to adverse climatic events, modesty, earliness, ease of calving, low birth weight of calves and good maternal instincts of cows. The main economic indicator of suckler cows rearing is the number of born and weaned calves. Production of twins is possible way to improve these indicators. The aim of this thesis was to compare the growth potential of twins of Aberdeen Angus beef breed with individuals who were born as singletons. The work is focused on comparing the weight at birth, in 120 and 210 days of age. Most twins in the population of Aberdeen Angus breed reared in the Czech Republic in 2009-2013 was born in 2012 - 9.44 % (270 pcs.), the least in 2013 - 6.67 % (184 pcs.). The incidence of twins by birth order increased from 1 to 3 birth order (78, resp. 130, resp. 131 pcs.) as further increasing order resulted into decline of twin's incidence. The difference in weight between twins and singletons at birth was 5.81 kg, in 120 days 28.32 kg and 37.32 kg 210 days (P 0.001). Differences within twins by sex were for bulls and heifers statistically significant (P 0.001). The relationship between weight at birth and calving ease was also investigated - in the case of twins relationship was not statistically significant (P> 0.05). The lowest mortality rate of twins was in 2013 - 8.70 % (16 pcs.), the highest mortality rate was in 2012 36.67 % (99 pcs.). Most twins were born in 2011 (179 pcs.), whereas least twins were born in 2010 (160 pcs.).
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The effect of pyrethroid based pesticides on fish
RICHTEROVÁ, Zuzana
Pyrethroids are ones of the most used pesticides worldwides. The widespread use and high stability of pyrethroids lead to the assumption of that their occurrence in the environment could be quite frequent. They can reach water ecosystem as pollutants. Residues of pyrethroids are not only detected in the water column, but also in sediments and in fish tissues. The first study was devoted to the product Nexide containing 60 g.l-1 of active substance gamma-cyhalothrin. Tested Nexide concentrations were 5, 25, 50, 100, and 250 µg.l-1. Early life stage test was used.Common carp (Cyprinus carpio) was tested. There were significant mortalities in all concentrations except the lowest concentration during the trial. The lowest concentration tested 5 µg.l-1 only caused a slightly increased mortality. This lowest concentration influenced the growth in length and weight negatively, decelerated ontogenetic development, and made the body surface of the individuals darker. Histopathology of individuals from this concentration revealed dystrophy in liver. Examination of kidney, intestine and gills did not show significant histopathological differences compared with control. The evaluation of selected parameters of oxidative stress demonstrated a significantly higher activity of detoxification enzyme glutathione-S.transferase (GST) and a significantly lower activity of defensive enzyme glutathione peroxidase (GPx) compared with the control group. The other examined parameters of oxidative stress such as catalase (CAT), glutation reductase (GR), and lipid peroxidation determined by using the thiobarbituric acid-reactive substances (TBARs) were comparable to the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Nexide in the given concentration leads to dysbalance of defensive enzymes. The second study was devoted to the product Cyperkill 25 EC containing 250 g.l-1. Tested Cyperkill 25 EC concentrations were 7.2, 36, 72, 144, and 360 µg.l-1. The procedure of the trial was the same as the preceded one. There were 100% mortalities in all concentrations except the lowest concentration during the trial. The lowest tested concentration 7.2 µg.l-1 allowed 90% of individuals to stay alive till the end of experiment. The lowest concentration influenced the growth in length and weight negatively and decelerated ontogenetic development compared with the control. Any individual exposed to this concentration did not reach juvenile stage until the end of the trial. Dark pigmentation was visible in 68% of these exposed individuals on the last day. Similar darkening was visible in individuals from higher concentrations shortly before death too. Histological examination did not revealed significant changes in intestine, liver, kidney, and gills compared with the control group. Evaluation of selected parameters of oxidative stress demonstrated significantly lower activities of GST, GR, and GPx. Activities of CAT and TBARS were comparable with the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Cyperkill 25 EC in the given concentration could induce oxidative stress and interfere with the activities of antioxidant enzymes. The presented thesis summarises actual data about pyrethroids and their influence on fish. The demonstrated effects confirm high susceptibility of early developmental stages of fish to tested pesticides. When interpreting the results, we have to take into account the fact that studies showed this risk even on single pyrethroid substances. But water organisms are exposed to many other more or less toxic products and substances in a real environment. These xenobiotics could react with each other and their mixture could even potentiate negative effects. The performed studies also clearly show the significant differences in the sensitivity of embryonic and embryolarval tests.
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Effect of water deficit on the energy content of individual plant organs in selected species of leafy vegetables
Šimůnková, Petra ; Hnilička, František (advisor) ; Česká, Jana (referee)
This work presents the impact of water deficit on dry mass production and photosynthetic accumulation of energy on every single plant´s organs of selected plant species. As a chosen experimental material were taken plants of Spinacea oleracea "Matador" sort and Tetragonia tetragonioides.
Spinacea oleracea and Tetragonia tetragonioides plants were grown in containers 11x11cm large in mixture of garden substrate A with silicon sand (2:1) in 2 variations placed in FAPPZ greenhouses. One variation was for control and second was exposed to stress reaction. Control variation had been watered for whole time of experiment with 50ml of water 2 times per week. Stressed one had been watered after puncturing and 6. taking until the end of experiment.
During the plant´s ontogenetic evolution had been monitored dry mass´s weight on singular plant´s organs. With usage of burnt calorimetry method had been monitored capacity of photosynteticaly accumulated energy in dry mass. Quantity of netto energy had been found out by dry burning adiabatic calorimeter LAGEST MS 10A.
From the gained results emerge, that plants of Tetragonia tetragonioides have 2 times higher weight growth apart from Spinacea oleracea. Control plants of Tetragonia tetragonioides have higher weight by 25,5% (123 mg) compared to the plants growing in water deficit. Likewise higher weight of roots had been observed on control plants (41 mg) compared to the stressed plants (40 g). Control spinach plants had higher weight of above-ground biomass by 19,5% compared to the stressed ones (43 mg). Roots of control plants had lower weight compared to stressed variant by 2960 mg.
From the results emerge, that plants of Tetragonia tetragonioides have higher weight of roots dry mass and above-ground biomass compared to the Spinacea oleracea plants.
Burning heat without ashes was on above-ground biomass of control plants Tetragonia tetragonioide on 15,96 kJ.g-1 level and on stressed plants 14,93 kJ.g-1 . In case of Spinacea oleracea plants was amount of control plants energy 14,98 kJ.g-1 and on stressed 14,21 kJ.g-1.
Burnt heat without root ashes of Tetragonia tetragonioides control plants was higher by 1.11 kJ.g-1 then on stressed ones (13,19 kJ.g-1). In case of Spinacea oleracea plants was amount of roots energy on control plants 11,90 kJ.g-1 and on stressed 11,72 kJ.g-1 .
Based on measurement were verified differences between species in reaction on water deficit and observed characteristic. From measuring emerged, that Spinacea oleracea plants are more sensitive on effect of water deficit compared to the Tetragonia tetragonioides
plants, which is more tolerant. Next thing observed is, that there are differences in amount of dry mass and rate of burnt heat in relation on effect of water deficit.
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Plant microclimate control
Jelínek, Jan ; Neděla, René (advisor) ; Papežová, Stanislava (referee)
This bachelor thesis discusses the management of a microclimate environment inside an
enclosed box. The aim of the work is to propose and implement a program for the
automized control of the microclimate. Microclimate management is ensured by a PLC
(Programmable Logic Controller) system utilizing the program Mosaic. Radishes
(Raphanous sativus L.) were chosen as the test plant. Optimal microclimate conditions for
the chosen test plant were achieved by programming the management system. The system
is easily adaptable, meaning endless different species of plants can be grown properly.
Healthy plant growth indicates the correct parameters of the microclimate.
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Gas exchange characteristics in relation to genotypes in spinach (Spinacia oleracea) under water stress
Helebrantová, Aneta ; Hnilička, František (advisor) ; Pazderů, Kateřina (referee)
The bachelors dissertation was compiled on theme of: Gas exchange characteristics in relation to genotypes in spinach (Spinacia oleracea) under water stress.
Spinach (Spinacia oleracea) is, similarly as the others leaf vegetables, difficult crop in terms of providing the sufficient level of moisture, therefore the attention is drawn to the varieties of spinach which are resistant to the water stress. Thus the target of cultivation is to find plant which will be resistance to influence of the water stress.
In climabox of department of botanics and physiology was founded experiment with three species of spinach: Misano F1, Monores a Matador. The temperature mode was set to 21 °C during the day and 17 °C during the night. The light mode was set to 16 hours of light and 8 hours of dark per day. Maximum light level in climabox was 800 micromole. The plants were cultivated in 4 recurrences, diagram of experiment is involving two variants: control and stress. The plants in control variant were cultivated in substrate, which was irrigated during the whole time of experiment by 250 ml of water. For the plants in stress variant the supply of water was suspended for 10 days and the substrate was naturally continuously dehydrating. After 10 days the water supply was restored (rehydration) for plants in stress variant, same as level of control variant. The observation was made for the speed of gas exchange (photosynthesis and transpiration) in two-day interval. The speed of gas exchange was measured on leaf area with infrared gasometric gas analyzer Lpro+ (ADC Bioscientific, Hodeson, UK). Measured was conducted in morning hours according. Based on the measured values of photosynthesis and transpiration we calculated water usage effectiveness (WUE).
Based on obtained results is evident that the highest average speed of photosynthesis in control variant was observed at variety of Monores (12,10 micromole CO2.m-2.s-1) and lowest at variety Misano (11,58 micromole CO2.m-2.s-1). The highest average speed of photosynthesis in stress variant was measured at variety of Matador (9,43 micromole CO2.m-2.s-1) and lowest at variety Monores (8,76 micromole CO2.m-2.s-1). There was observed decrease of photosynthesis for each of variety during the water stress.
The highest average values of transpiration were observed at variety Monores (1,97 mmol H2O.m-2.s-1), lowest at variety Matador (1,68 mmol H2O.m-2.s-1). Stressed variety Misano reached level of photosynthesis 1,82 mmol H2O.m-2.s-1. Control variety Matador reached speed of transpiration 1,54 mmol H2O.m-2.s-1 and variety Monores 1,85 mmol H2O.m-2.s-1.Variety Misano was on same level of control variant as stress variant.
The most sensitively reacted variety Monores, which usage of water was 4,43 (10-3). Variety Matador managed the stress well, the usage of water was 5,60 (10-3).
Obtained results confirmed hypothesis of genotype differences depending on water deficit, thus there are differences between gas exchange and WUE in control and stress plants.
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