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Vývoj, optimalizace a validace analytické metody na stanovení kyseliny chikorové a dalších derivátů kyseliny kávové pomocí LC/MS
PAZDERKOVÁ, Nela
This thesis deals with the development, optimization, and validation of an analytical method for the determination of chicoric acid and other caffeic acid derivates using LC/MS. First, the development and optimization of the analytical method was conducted. The following parameters were selected for the optimization: mobile phase composition and flow rate injection volume capillary temperature, voltage S-lens and F-lens voltage, ion injection time, product ion selection, and collision energy. Then, the analytical method was successfully validated and tested for quantitative analysis in a range of Echinacea food matrixes - tea, tablet, and tincture.
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Determination of CDT by capillary zone electrophoresis method.
BUREŠOVÁ, Kristýna
Alcohol abuse is part of very serious social problems with many social and economic impacts in developed countries. Chronical alcohol abuse may be in many cases very complicated to diagnose and good cooperation between clinician and laboratory workspace is fundamental. Routine diagnosis of chronic alcohol abuse is nowadays based on GGT and MCV determination and AST/ALT ratio. Recently, as a marker with the highest diagnostic validity is considered Carbohydrate Deficient Transferrin (CDT). The transferrin molecule consists of two polysaccharide chains where on each chain could be attached up to four sialic acids (so-calledsialization). However, sializationmay be after two weeks of increased alcohol intake significantly reduced. Detection of these less sialized transferrin molecules, known as CDTs, can be used to diagnose chronic alcohol abuse. Unfortunately, informations from literature about the diagnostic validity of CDT are highly variable. CDT validation differs among women and men and the highest diagnostic validity is achieved during determination in combination with other markers. The experimental part was done on the MINICAP instrument, which works based on the principle of capillary zone electrophoresis. Measurements of control materials and mixed serum patients were performed. From measured values, the necessary statistical values were calculated, such as arithmetic mean, standard deviation and coefficient of variation. Based on the calculations, the validation of the method on the MINICAP instrument was then performed and evaluated for CDT determination.
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