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Milk composition changes after fortification milch cow feed with selected additive
Tůmová, Michaela ; Hrstka, Miroslav (referee) ; Křížová,, Ludmila (advisor)
This thesis is dealing with effect of supplement limiting aminoacid histidin, that was applied as duodenal infusion, on yield and composition of high production dairy cows milk. The theoretical part describes overview about milk composition and metabolism of dairy cows. There are described nutrition requirements of dairy cows for esential aminoacids. In this part are also presented principles of analytical methods used for analysis of milk. For determination of basic components of milk were used infrared spectroscopy and enzymatic-conductometry. Aminoacid profile of casein was determined by ion exchange liquid chromatography. In the experimental part is described method of experiment. There is described technique of acid and oxidative hydrolysis of lyofilized casein and technique of determination aminoacids in this hydrolyzated casein. Results of analysis were interpreted and discussed with literature. This diploma thesis was made as a cooperation between the Institute of Food Science and Biotechnology, the Faculty of Chemistry, University of Technology, Brno and the Research Institute for Cattle Breeding, Department of Animal Physiology and Nutrition, Pohorelice.
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Determination of caseins by capillary electrophoresis
Mičíková, Ivana ; Zemanová, Jana (referee) ; Vespalcová, Milena (advisor)
The diploma thesis, Determination of the caseins by the capillary electrophoresis, is dealing with a creating of the analytical method for a determination of the caseins in cow‘s milk. The capillary electrophoresis was used as a separative method. The developed procedure was optimalized and verified on standards of the caseins and the real samples. The theoretical part gives an information about the compound of cow's milk, caseins, their usage in the food industry and the non-food technologies. There are mentioned some further nutritional factors, whose application in the cattle nutrition can affect the milk composition. The experimental part describes the procedure of the caseins isolation from milk, the used separative system and the determination of the caseins in the lyophilisated samples of milk. The separation was performed in two untreated fused-silica capillaries. Both of them had the same internal diameter: 50 m. The total length of the first capillary was 96 cm (71 cm of effective length) and of the second used capillary 125 cm (effective length 100 cm) The phosphate buffer (10 mM, pH 2,5) served as a basic electrolyte. The diploma thesis has arisen on the basis of the cooperation of the Institute of Food Science and Biotechnology, the Faculty of Chemistry, Brno University of Technology and the Research Institute for Cattle Breeding, Ltd., Department of Animal Nutrition Physiology in Pohořelice.
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Analysis of cow milk caseins by capillary electrophoresis
Dvořáková, Irena ; Zemanová, Jana (referee) ; Vespalcová, Milena (advisor)
The diploma thesis is dealing with the determination of caseins in lyophilised samples of milk using the method of capillary electrophoresis (CE). This method was optimised and verified by Ivana Micikova in her diploma thesis in 2009. The theoretical part of the diploma thesis gives information about the chemical compound of cow's milk. Caseins and their usage in the food and non-food industries are described in a separate unit. Impact of both the nutritional and non-nutritional factors on the composition of the milk is discussed further. The description and the principles of the CE and a list of the methods of CE used in the last several years are stated at the end of this part. The experimental part is focused on the preparation of the solutions, process of casein isolation and its analysis. It summarises the setting of the experiment, sampling and sample processing and the composition of the feeding mixtures. The diploma thesis has arisen on the basis of the cooperation of the Institute of Food Science and Biotechnology, the Faculty of Chemistry, Brno University of Technology and the Research Institute for Cattle Breeding, Ltd., Department of Animal Nutrition Physiology in Pohorelice.
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Analýza úrovně výživy a vybraných kvalitativních ukazatelů produkce mléka
KAZDA, Miloslav
The diploma thesis focuses on the analysis of the nutritional level of dairy cows and its influence on selected qualitative indicators of milk production. The aim of the work is to carry out a comprehensive assessment of the impact of feed ration and its composition on the quality and quantity of milk production, with an emphasis on identifying optimal nutritional conditions for achieving the highest utility and ensuring good health and living conditions for dairy cows. In the first part of the work, a theoretical review of current knowledge about dairy cow nutrition and milk quality was carried out, focusing on energy and nutrient needs of dairy cows, quality and optimal representation of feed, individual phases of lactation, milk quality, microbiological and hygienic value of milk, and factors affecting its quality. In the second part of the work, an analysis of the nutritional level in the selected breeding was performed. Mixed feed rations and individual components contained in them were evaluated here. Furthermore, utility and qualitative indicators of milk (fat content, protein, lactose, somatic cells, and total number of microorganisms) were assessed.
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Analýza úrovně výživy a vybraných ukazatelů v daném zemědělském podniku
FALTUSOVÁ, Tereza
The nutrition of dairy cows affects their yielding, health condition, fertility, and the overall economic results of the holding. Essential for the nutrition of dairy cows is the quality of the roughage, which affects the cost of feeding ration of the dairy livestock. This work evaluated the quality of fodder and its influence on selected indexes of a chosen agricultural holding during the year 2020. The evaluation concerned a Holstein cattle herd with an average quantity of 447 dairy cows. As mentioned above, the feeding ration affects the yielding of dairy cows. With the right amount of nutrients (nitrogen substances, energies, carbohydrates, fibre, fats, and mineral substances) in the feeding ration we might achieve higher amounts of components in milk (fat, proteins, and lactose). If the feeding ration is suitably designed, health issues (metritis, mastitis, and limping) do not occur in the herd. The fodder quality, yielding, and health condition of the herd affect the economics of the holding. The base for the feeding ration was roughage, which constituted 54 % of the feeding ration. Specifically, corn silage and protein silage constituted 27 % of the fodder dry mass. The milk components showed an average amount of 4,11 % of fat and 3,45 % of protein. The costs of 1 l of milk were in the year 2020 9,52 Kc/1 l on average and the purchase price of milk was 8,69 Kc/1 l on average.
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Optimalizace výživy a analýza vybraných ukazatelů produkce mléka
PAČESOVÁ, Kristýna
The fundament of this diploma thesis represents the nutrition of dairy cows which is affected by the quality of conserved forage. Within the evaluation of nutritional value were also comprised the factors of housing and watering of dairy cows. All indicators were observed during the years 2019 and 2020, under operating conditions. The optimization of the feed rations was performed, based on the feeding program assessment, to increase the milk yield. During both observed years, the main type of feed was corn silage. In 2020, 55 % of forage and 43 % of the total feed was represented by corn silage. Mixed feed rations for lactating dairy cows contained less dry matter (45 % in 2019 and 43.58 % in 2020), as well as a lower concentration of energy. In the context of raising productivity, it was proposed to increase the concentration of NEL in feed rations and keep the optimal values of nitrogenous substances (in the year 2020 - 162.5 g/kg of dry matter) and fibre (NDF). After the evaluation of milk yield, it was statistically proven the influence of the season on the fat and protein content in milk. Between the years 2019 and 2020, the average daily milking efficiency increased by 1.9 L/one dairy cow. In 2019, the price per one litre of produced milk was 8.43 CZK, in 2020 the cost was reduced to 7.52 CZK. Both observed years were profitable, in 2020 the profit increased by CZK 7,368 per one year and one dairy cow. The company had a lower cost per one dairy cow in a comparison with the average cost in the Czech Republic. Subsequently, the new feed ration resulted in a daily yield of 34.43 L/one dairy cow. The feed ration was enriched by corn in the form of CCM and molasses. On the contrary, the "GPS" feed was discarded.
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Základní aspekty výživy dojnic v průběhu laktace
KAZDA, Miloslav
This bachelor's thesis deals with the nutrition of dairy cows during lactation. There is characterized feeding of dairy cows in the whole meantime. Work contains of recommended feed rations with optimum amount nutrients during phases of lactacion. A brief description is also given to the nutrients, feeds and diseases caused by incorrect nutrition.
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Analýza farmy dojnic za účelem kontroly výživy, metabolismu a zdraví dojnic ve vztahu k užitkovosti
Šiklová, Veronika
The aim of the final thesis was to control the management of dairy cows breeding, nutrition, health and milk production in the chosen company. The next aim was to recommend measures in the order to improve the business situation, to realize some of them and compare results. Literary part is focused on the characteristic of nutrition of calves, heifers and dairy cattle. Methods how to control nutrition and metabolism with using feed mixture, excrements, ruminant, body condition, control of milk production and a content of milk components analysis are described. Practical part includes a description of the company, an analysis of the situation in April 2018 and recommended improvement measures. The implemented measures on the farm in summer 2018 are also described. The breed is characterized by data such as number of cattle, results of reproduction and health, average yields in last years and milk components. Furthermore, the changes that took place on the farm during the reporting period are described. The weights of two groups of calves with different nutrition were compared and evaluated (the group of calves fed with milk and starter from the birth vs. the group of calves fed only with milk). At the age of three months, calves fed with milk and starter had significantly higher weight compared to the group of calves which were fed with starter during weaning period. The milk yield was inconclusive (p = 0.06) after the realization of the measures. However, there were significant changes in milk components — protein, fat and urea content increased. The comparision of milk yield and milk components of the Czech Fleckvieh’s and Holstein’s breed was evaluated. Holstein dairy cows had a higher milk yield with lower somatic cells. On the other hand milk of Czech Fleckvieh’s cows contained more protein and fat. Other analyses showed, that cows are getting fat at the end of lactation and during dry period. Suspicions of acidosis and insufficient homogeneity of feed mixture were evaluated (because of the different consistency of feces of dairy cows in the same group, the content of the raw elements was high in feed ration, the problem of separation of feed mixture components during transport by belt conveyors was proven).
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Vliv bílkovinných koncentrátů na obsah aminokyselin v mléce dojnic
Hubálková, Alžběta
The aim of the experiment was observing the effect of protein concentrate addition to the amino acid composition in milk from dairy cows of the holstein breed in ecological agriculture. There were 20 dairy cows selected for the experiment and divided into two groups. Both groups have the same basic feed dosage consisting of maize si-lage, clover-grass haylage – first cut, grass haylage – first cut, winter wheat and spring barley. The control group of dairy cows (n = 10) was fed only with a basic feed dosage. There was added protein concentrate to the control feed dosage for the experimental group of dairy cows, in an amount of 1 kilogramme per unit per day. The protein concentrate contained soybean, linseed and sunflower cakes. Whole experiment lasted 30 days. Sampling of milk for the determination of amino acid concentration in milk was taken regularly every tenth day. The addition of protein concentrate increased the concentration of aspartic acid and glycine (P < 0.05), the effect of the other monitored amino acids were not statistically significant. According the results, it is likely that the addition of protein concen-trate may affect the concentration of some amino acids in milk of dairy cows.
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