National Repository of Grey Literature 6 records found  Search took 0.01 seconds. 
Creation of electrochemical sensors for DNA analysis
Šílený, Jan ; Adam, Vojtěch (referee) ; Hubálek, Jaromír (advisor)
The objective of this work was creation of an electrochemical sensor for DNA analysis using thick layer technology. Development of sensors is described from the design face with particular steps of realization along with theory. Measurements with results and evaluation of possible application in common use are described.
Operation and optimization of a capacitive deionization unit
Vespalec, Jan ; Mergl, Václav (referee) ; Repková, Martina (advisor)
This bachelor thesis is focused on the extension of the information available so far on capacitive deionization within the framework of research activities in the field of separation methods. In the theoretical part, the laws of deionization, factors influencing the course of deionization, possible configurations of the capacitive deionization model, electrode corrosion issues, practical applications and future developments of capacitive deionization are discussed. The experimental part describes the capacitive deionization unit located at the FCH BUT, the procedure of electrode replacement, calibration of pH and conductivity probes and the optimal setting of factors influencing the course of deionization. In this bachelor thesis, a series of measurements were carried out to find the optimal setting of the unit.
Effect of Genotoxic 2-Nitrofluorene and Its Metabolites on DNA In Vivo and Possibilities of Investigation of this Effect Using Electrochemical DNA Biosensors In Vitro
Stávková, Klára ; Vyskočil, Vlastimil (advisor) ; Fišer, Radovan (referee)
Presented Bachelor Thesis is targeted on the topic of hazardous 2-nitrofluorene (NF), which is known as one of the markers of the presence of nitrated polycyclic aromatic hydrocarbons. This Thesis summarizes the current knowledge about the formation of this xenobiotic, its occurrence and transport in living and working environment, and about its negative effects on living organisms, especially on their DNA. These findings obtained upon the in vivo investigations are compared in this Thesis with information obtained using electrochemical DNA biosensors, which represent very promising in vitro alternative to the study of processes proceeding in living organisms upon the interaction of their DNA with the xenobiotic. NF is mainly released to the atmosphere through the exhaust gases of diesel and gasoline engines. It is adsorbed onto solid particulate matters, through which it is spread over long distances and can enter the air passages of animals. Through an atmospheric fall-out, NF contaminates waters and soils, where it is transported together with other nutrients and food to plants and animals; by consummation of such organisms, it can finally enter the body of higher animals and the human. It has been shown that NF is a chemically stable substance. On the other hand, it is metabolized intensively...
Operation and optimization of a capacitive deionization unit
Vespalec, Jan ; Mergl, Václav (referee) ; Repková, Martina (advisor)
This bachelor thesis is focused on the extension of the information available so far on capacitive deionization within the framework of research activities in the field of separation methods. In the theoretical part, the laws of deionization, factors influencing the course of deionization, possible configurations of the capacitive deionization model, electrode corrosion issues, practical applications and future developments of capacitive deionization are discussed. The experimental part describes the capacitive deionization unit located at the FCH BUT, the procedure of electrode replacement, calibration of pH and conductivity probes and the optimal setting of factors influencing the course of deionization. In this bachelor thesis, a series of measurements were carried out to find the optimal setting of the unit.
Effect of Genotoxic 2-Nitrofluorene and Its Metabolites on DNA In Vivo and Possibilities of Investigation of this Effect Using Electrochemical DNA Biosensors In Vitro
Stávková, Klára ; Vyskočil, Vlastimil (advisor) ; Fišer, Radovan (referee)
Presented Bachelor Thesis is targeted on the topic of hazardous 2-nitrofluorene (NF), which is known as one of the markers of the presence of nitrated polycyclic aromatic hydrocarbons. This Thesis summarizes the current knowledge about the formation of this xenobiotic, its occurrence and transport in living and working environment, and about its negative effects on living organisms, especially on their DNA. These findings obtained upon the in vivo investigations are compared in this Thesis with information obtained using electrochemical DNA biosensors, which represent very promising in vitro alternative to the study of processes proceeding in living organisms upon the interaction of their DNA with the xenobiotic. NF is mainly released to the atmosphere through the exhaust gases of diesel and gasoline engines. It is adsorbed onto solid particulate matters, through which it is spread over long distances and can enter the air passages of animals. Through an atmospheric fall-out, NF contaminates waters and soils, where it is transported together with other nutrients and food to plants and animals; by consummation of such organisms, it can finally enter the body of higher animals and the human. It has been shown that NF is a chemically stable substance. On the other hand, it is metabolized intensively...
Creation of electrochemical sensors for DNA analysis
Šílený, Jan ; Adam, Vojtěch (referee) ; Hubálek, Jaromír (advisor)
The objective of this work was creation of an electrochemical sensor for DNA analysis using thick layer technology. Development of sensors is described from the design face with particular steps of realization along with theory. Measurements with results and evaluation of possible application in common use are described.

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