Home > Search Results: keywords:"ubikvitin-proteazomový systém"
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
The role of Fbxo38 ubiquitin ligase in mouse spermatogenesis
 Zobalová, Eliška ; Čermák, Lukáš (advisor) ; Stopka, Pavel (referee)
Cullin-dependent ubiquitin ligases are responsible for the regulation of most cellular processes. Despite their mutated forms being the cause of many human diseases, their physiological roles are not sufficiently described. In the presented results, we focused on the physiological role of ubiquitin ligase SCFFBXO38 (SKP1-CULLIN1-FBXO38), whose mutated forms are responsible for the progression of distal neuropathy. Preparation of mouse model deficient in FBXO38 revealed that homozygous pups were born in a lower than expected ratio. Animals were growth-retarded, both at the level of the whole organism and individual organs, especially the liver and testes. Males with a deletion in the Fbxo38 gene had significantly lower reproductive capacity, which was associated with lower production of mature sperm and pathological changes in the structure of seminiferous tubules. We found that the FBXO38 protein is functionally expressed in Sertoli cells responsible for regulating spermatogenesis and seminiferous tubules integrity. Detailed analysis of spermatogenic populations revealed a defect at the level of spermatocyte differentiation. The dynamics of this differentiation depend on the hematotesticular barrier functional integrity formed by the intercellular junctions of Sertoli cells. We confirmed that the...
Detailed record
Narušení metabolismu proteinů a jeho efekt na růst rostlin
 Luklová, Markéta
Recent years have shown that plant hormones operate through circuits in which targeted, conditional turnover of regulator proteins via proteasome are of critical importance. Thus, a disruption of the ubiquitin-proteasome system (UPS) would have a significant impact on plant growth and development. This thesis entitled Protein metabolism disruption and its effect on plant growth reviews the mechanisms of proteasome-mediated targeted degradation and analyzes its role in plant hormone cytokinin signaling. The experimental part is divided into two sections (i) growth response analyzes and (ii) an LC-MS profiling of diurnal variations in transgenic Arabidopsis seedlings RV 86-5 carrying modified inducible ubiquitin that prevents protein degradation via the UPS. The results present evidence that an intricate balance between the UPS and cytokinin modulates the growth response output of cytokinin signaling. On the proteome level, an LC-MS profiling identified >4,000 proteins, 61 of which were previously identified in response to proteasome inhibition.
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English