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První údaj o resistenci Digitaria sanguinalis vůči atrazinu v České republice
 Salava, J. ; Chodová, D. ; Kočová, M. ; Krahulec, František
A survey on the occurrence of weeds surviving treatment with atrazine was carried out at the railways in the Czech Republic in 2000-05. Large crabgrass was widely distributed at the surveyed railway junctions. Seeds of large crabgrass were collected from plants growing in the surroundings of the railway station Prague-Bubny in 2003 and 2004. Resistance or susceptibility to atrazine was verified by the whole-plant response and chlorophyll fluorescence assays. A region of the psbA gene encoding D1 protein of Photosystem 2 was amplified by PCR and sequenced to determine the molecular basis for the atrazine resistance. It was found out that the resistance in the Prague-Bubny large crabgrass biotype was conferred by a glycine for serine substitution at residue 264 of the Dl protein of Photosystem 2. There was an excellent correspondence between the presence of the mutation and phenotypic resistance to atrazine of individual plants.
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