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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Genetically modified products from feed in kidneys of broilers
 POSPÍŠIL, Miroslav
This bachelor thesis gives an overview of managing detection of genetically manipulated products from chicken feed. Kidneys of chickens which were fed by feed that has been genetically modificated were subject to examination. Two GM plants were necessary for the examination, one of them being resistent Bt-maize, the other one herbicide-tolerant Roundup Ready soya. Both plants were used as additives in feed mixture for poultry. Detection of prospective trangenes was realized by means of molecular-genetic methods {--} PCR and electrophoresis. Ascertained risks for consumers will be evaluated upon the results of the thesis.
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The role of histone modifications and gene expression in mouse spermatogenesis
 Křivánková, Klára ; Mihola, Ondřej (advisor) ; Jansa, Petr (referee)
The production of haploid sperm is a precondition for sexual reproduction of males. PRDM9 protein is a histone methyltransferase which localizes sites of meiotic recombination in many mammals. Mouse males of the C57BL/6J (B6) strain deficient for Prdm9 (Prdm9-/- ) are sterile, while Prdm9-/- males of PWD/Ph (PWD) strain have reduced sperm count. The comparison of the distribution of trimethylation of histone 3 on lysine 36 (H3K36me3) in genome of Prdm9-/- males of these two strains will help to determine the role of this epigenetic modification on meiotic recombination and fertility of Prdm9-/- males. The second part of this thesis is focused on transgenic males. Male offspring from the first generation of B6 female and PWD male crosses (B6PF1) have reduced fertility parameters due to incompatibility of Prdm9 alleles. The fertility parameters of B6PF1 hybrids carrying CHORI-34-289M8 or RP24-346I22 transgene are even lower. The candidate gene, which participates in the reduction of fertility of the transgenic B6PF1 hybrids, was determined as the proteasome subunit encoding gene Psmb1, because its relative transcription level best correlates with sperm count. The reason of lowered fertility thus might be a defect in proteasome assembly. The investigation of the fitness of transgenic animals is...
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The molecular analysis of transgenic \kur{Lycopersicon esculentum} plants harbouring incorporated pmi gene for phosphomannose isomerase
 PŘIKRYLOVÁ, Pavla
The aim of this study was to analyze number of incorporated T-DNA harbouring pmi and nptII transgenes and expression of the pmi gene in tomato transformants using Southern blot and Northern blot methods. The presence of a functional PMI protein was assesed using PMI-assay with Chlorophenol red dye. Mannose selection of tomato seeds was established and segregation patterns in T1 progeny were studied.
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