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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Konštrukcia a charakterizácia mutantných foriem cytokinínového receptora CRE1 AHK4 z Arabidopsis thaliana
 Pacurová, Marika
The CRE1 / AHK4 plant receptor produces after cytokinin binding a signal transferred to the cell nucleus where it regulates the transcription of target genes. The functionality of the receptor is retained after expression in the Escherichia coli KMI001 which is then used for the study of the receptor, where ß-galactosidase activity is detected in response to cytokinins. The "Construction and Characterization of Arabidopsis thaliana CRE1 / AHK4 Cytokinin Receptor Mutant Forms" describes the principle of mutagene-sis of a commercially available kit and approach published in the literature, summarizes the function of the AHK4 receptor, describes its signal pathway after expression in the bacterial strain KMI001 and characterizes its mutant forms. The experimental part focuses on the PCR mutagenesis carried out by the two methods including the design of mutagenic primers containing the desired mutation and performing the transformation of the mutant construct into Escherichia coli strain KMI001, as well as the β-galactosidase activity test of the mutant variants CRE1 / AHK4 receptor in the presence of the trans-zeatin hormone concentrations. The results indicate that mutations L437F, L437G, L437I, L437W and L437Y cause increased activity at low level of the trans-zeatin concentrations compared to wild-type CRE1 / AHK4 receptor, but the effect of the L437H mutation causes loss of this activity.
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The impact of constitutive overexpression of trans-zeatin O-glucosyltransferase gene in tobacco on photosynthetic pigments and gas exchange during water stress
 Haisel, Daniel ; Pospíšilová, Jana ; Nováková, Marie ; Vaňková, Radomíra
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