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National Repository of Grey Literature

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Isolation of photosystem II particles and their characterization Svitačová, Denisa ; Pekař, Miloslav (referee) ; Venerová, Tereza (advisor) This master's thesis deals with the isolation and characterization of spinach thylakoid membranes and photosystem II particles. Based on the research, two procedures were chosen for the isolation of spinach thylakoid membranes and photosystem II particles with an emphasis on preserving their native properties. Subsequently, the characterizations were carried out, focusing on the spectroscopic properties of the isolated samples. UV-VIS spectrometry was used to determine the ratio of chlorophyll a to chlorophyll b in samples of thylakoid membranes and photosystem II particles. The success of the isolations was confirmed by fluorescence methods. The excitation and emission spectra of the samples were measured by the steady-state fluorescence spectroscopy, and the fluorescence lifetimes of the samples of thylakoid membranes and photosystem II particles were determined by time-resolved fluorescence. The isolated samples were afterwards visualized with a fluorescence microscope with a CCD camera and the FCS technique. Detailed record

Stereologická metoda pro odhad povrchu tylakoidů chloroplastu Kubínová, Lucie ; Kutík, J. The stereological method using “local vertical windows” applied to the estimation of thylakoid surface area in the chloroplast volume, based on evaluation of chloroplast electron micrographs is presented. The method is demonstrated on the study of chloroplast ultrastructure in the leaves of plants of CE704 maize (Zea mays L.) line, developing in control and chilling conditions Detailed record

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