Home > Search Results: keywords:"tethering complex exocyst"
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Functional characterization of plant EXO70 exocyst subunit isoforms and their membrane targeting mechanisms
 Sekereš, Juraj ; Potocký, Martin (advisor) ; Jaillais, Yvon (referee) ; Ischebeck, Till (referee)
Vesicle tethering complex exocyst is one of the key regulators of the cell polarity and morphogenesis in eukaryotes. The complex interacts with the secretory vesicle, as well as plasma membrane, and facilitates formation of cis SNARE complex leading into fusion of the vesicle with target destination. Two of the eight exocyst subunits, the SEC3 and EXO70 are known to bind plasma membrane via protein and lipid interactors in Opisthokont model organisms. Genomes of angiosperm plants encode a surprisingly wide repertoire of EXO70 isoforms with over 20 present in both Arabidopsis and diploid tobacco genome. It has been proposed that different EXO70 isoforms would form parts of functionally distinct subtypes of the plant exocyst complex driving membrane trafficking to various membrane domains. Specific interactions of peripheral membrane proteins with particular membrane phospholipids largely contribute to targeting of cellular components to subcellular compartments and membrane domains. This thesis focuses on role of protein-lipid interactions in regulation of plant cell polarity and contributes to functional analysis of the plant EXO70 family diversity. We introduce the topic with the theoretical reviews summarizing role of protein-lipid interactions in establishing plant cell membrane domains at...
Detailed record
FUNCTIONAL ANAYSIS OF SELECTED EXO70 EXOCYST SUBUNITS IN PLANTS
 Kubátová, Zdeňka ; Kulich, Ivan (advisor) ; Grossmann, Guido (referee) ; Lichtscheidl-Schultz, Irene (referee)
Arabidopsis thaliana trichomes are large unicellular epidermal outgrowths with a specific development and intriguing shape, which makes them an excellent cell type for our research of cell polarization mecha- nisms. Cell polarity is essential for plant development and the exocyst complex is one of its key regulators. It is an octameric protein complex that mediates polarized exocytosis and growth by targeted tethering of secretory vesicles to the plasma membrane. Its EXO70 subunit functions as a landmark for exocytosis site and physically binds the target membrane through interaction with phospholipids. A remarkable multipli- cation of EXO70 subunit paralogs in land plant genomes is well documented, but the functional diversity of these paralogs remains to be described. In trichomes we revealed the specific role of the EXO70H4 paralog in secondary cell wall deposi- tion, especially in callose synthase delivery. We documented formation of a thick secondary cell wall during the maturation phase of wild type trichome development and a lack of it in the exo70H4 mutant. Moreover, we showed evidence for silica deposition dependency on callose synthesis. Further, we unveiled the formation of apical and basal plasma membrane domains, which differ in their phospholipid compo- sition and ability to bind...
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English