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Bioinformatic analysis of extremophillic bacteria with respect to their capability of polyester accumulation
 Bočková, Sabina ; Sedlář, Karel (referee) ; Obruča, Stanislav (advisor)
This bachelor thesis is focused on the bioinformatic analysis of extremophilic bacteria in the context of their ability to accumulate Polyhydroxyalkanoates (PHA) – microbial storage polyesters. Specifically, we conducted bioinformatic research using the online available databases BacDive and BLAST to identify new thermophilic and psychrophilic bacterial strains with genetic predispositions for PHA synthesis. The identification of these potential PHA producers was based on the presence of the phaC gene (using the phaC gene from the bacterium Cupriavidus necator as a template). Using the BLAST database, the optimal cultivation temperature was then verified for the selected candidates in the BacDive databse. Selected bacterial strains that contained the phaC gene and fit into the group of themophiles or psychrophiles wre thoroughly characterized through literature review. Based in the obtained data, two tables were cimpiled containing a total of 60 themophilic and 60 psychrophilic bacteria with genetic predispositions for the PHA synthesis. In the experimental part, five psychrophilic bacteria of the genus Massilia were selected and the presence of the phaC gene was exymined using polymerase chain reaction (PCR). The presence of the class I phaC synthase was confirmed in two other bacteria. In one strain, it was likely that DNA isolation in the required purity and quantity was not successful.
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