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Norway spruce chloroplast ultrastructure - heterogeneity within a needle.
Glanc, Natália ; Lhotáková, Zuzana (advisor) ; Kutík, Jaromír (referee)
6 Abstract Temperate forests serve as long term carbon storage and are affected by increasing carbon dioxide (CO2) concentration in the atmosphere. Norway spruce (Picea abies (L.) Karst.) is the most abundant conifer in the forests of the Czech Republic, therefore I studied the response of its photosynthetic aparatus to elevated CO2 concentration. The aim of my thesis was to analyze the impact of CO2 concentration on chloroplast ultrastructure in both shaded and exposed needles, focusing on the volume density of starch in the median cross-sections of mesophyll cell chloroplasts. The next aim of the study was to test whether the chloroplasts of the first subepidermal layer of mesophyll are representative for the whole needle with respect to starch volume density. The study was performed on eleven years-old Norway spruce trees that had been exposed to ambient or elevated concentration of CO2 for six years; the experiment had been carried out at the Bílý Kříž experimental station in the Beskids Mountains in cultivation chambers with automatically adjustable windows. First year needles of trees grown under abient (382-395ppm) or elevated (700 ppm) CO2 concentration were collected in October 2011. The needles were used to prepare ultrathin sections and the images of median chloroplast cross-sections were...
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Norway spruce chloroplast ultrastructure - heterogeneity within a needle.
Glanc, Natália ; Lhotáková, Zuzana (advisor) ; Kutík, Jaromír (referee)
6 Abstract Temperate forests serve as long term carbon storage and are affected by increasing carbon dioxide (CO2) concentration in the atmosphere. Norway spruce (Picea abies (L.) Karst.) is the most abundant conifer in the forests of the Czech Republic, therefore I studied the response of its photosynthetic aparatus to elevated CO2 concentration. The aim of my thesis was to analyze the impact of CO2 concentration on chloroplast ultrastructure in both shaded and exposed needles, focusing on the volume density of starch in the median cross-sections of mesophyll cell chloroplasts. The next aim of the study was to test whether the chloroplasts of the first subepidermal layer of mesophyll are representative for the whole needle with respect to starch volume density. The study was performed on eleven years-old Norway spruce trees that had been exposed to ambient or elevated concentration of CO2 for six years; the experiment had been carried out at the Bílý Kříž experimental station in the Beskids Mountains in cultivation chambers with automatically adjustable windows. First year needles of trees grown under abient (382-395ppm) or elevated (700 ppm) CO2 concentration were collected in October 2011. The needles were used to prepare ultrathin sections and the images of median chloroplast cross-sections were...
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The effect of elevated CO2 concentration and irradiation on quantitative parameters of mesophyll cells of Norway spruce
Kubínová, Zuzana ; Albrechtová, Jana (advisor) ; Radochová, Barbora (referee)
KUBÍNOVÁ, Zuzana. The effect of elevated CO2 concentration and irradiation on quantitative parameters of mesophyll cells of Norway spruce. Prague, 2010. 74 p. Master's degree thesis. Faculty of Science, Charles University in Prague. Abstract The aim of the present thesis was to choose and adjust a suitable methodology for counting particles in 3D space, which would be suitable for unbiased estimation of chloroplast number in needle mesophyll cells. The disector method was used for the first time to determine the number of chloroplasts. This method enables unbiased estimation of chloroplast number in needle volume from optical sections captured from fresh free-hand sections by confocal microscope. The sections did not need any pre-processing. Another aim was to compare selected photosynthetic and anatomical characteristics of sun and shade Norway spruce needles, which were grown under different CO2 concentration. The trees were grown for eight years in ambient (during the experiment increasing from 357 up to 370 µmol CO2 ∙ mol-1 ) CO2 concentration or elevated (700 µmol ∙ mol-1 ) CO2 concentration in special glass domes on an experimental research site of the Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic at Bílý Kříž in Moravskoslezské Beskydy mountains. The sun needles...
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