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Drugs Loading in Thermosensitive Micellar Gels
Smolková, Miroslava ; Pavliňáková, Veronika (referee) ; Chamradová, Ivana (advisor)
Presented diploma thesis deals with drug encapsulation of Sulfathiazole (STA) in thermosensitive hydrogel composed of amphiphilic fibrous molecules of copolymer poly(D,L-lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA). In the experimental part, the structure of the micelles which is dependent on the concentration of the default copolymer, was thoroughly described. The method of dynamic light scattering (DLS) was used to dimensionally characterize the micellar size in aqueous solution. At the same time, the Cryo-Transmission Electron Microscopy (Cryo-TEM) imaging technique confirmed the transition from spherical micelles to fibrous branched structures with a number of topological interactions. The change in viscoelastic properties of micellar hydrogel after addition of STA was studied by rheological analysis. The studied triblock copolymer appears to be a suitable tissue engineering material as a carrier in applications for targeted drug delivery and tissue regeneration.
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Voltammetric determination of sulfathiazole on boron doped diamond electrode
Zich, Mikuláš ; Fischer, Jan (advisor) ; Schwarzová, Karolina (referee)
This bachelor thesis is dedicated to study of the electrochemically active substance sulfathiazole. Sulfathiazole is an antibacterial agent added to animal feed. As a result, it contaminates soil, water bodies and food. A boron-doped diamond electrode was chosen as the working electrode for voltammetric determination. First, the optimal pH for the study of sulfathiazole in the range of 2 to 12 by the technique differential pulse voltammetry was determined and the optimal pretreatment of the electrode before measurements. Subsequently, the calibration dependence was measured. Sulfathiazole was successfully dissolved in water at a concentration of c = 1·10-3 mol l-1 and Britton-Robinson buffer was used as the electrolyte. As the optimal pH was determined pH 6,0. At this pH, a relative standard deviation of sr = 5% was achieved. Standart deviation of 5 % has been achieved when the working electrode was polished before each measurement. Under these conditions, was calibration dependence measured in concentration range from 0,1 to 100 μmol l-1 . Linear dynamic range was calculated between 0,1 - 20 μmol l-1 . Limit of quantitation was found to be 97 nmol l-1 .
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Drugs Loading in Thermosensitive Micellar Gels
Smolková, Miroslava ; Pavliňáková, Veronika (referee) ; Chamradová, Ivana (advisor)
Presented diploma thesis deals with drug encapsulation of Sulfathiazole (STA) in thermosensitive hydrogel composed of amphiphilic fibrous molecules of copolymer poly(D,L-lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA). In the experimental part, the structure of the micelles which is dependent on the concentration of the default copolymer, was thoroughly described. The method of dynamic light scattering (DLS) was used to dimensionally characterize the micellar size in aqueous solution. At the same time, the Cryo-Transmission Electron Microscopy (Cryo-TEM) imaging technique confirmed the transition from spherical micelles to fibrous branched structures with a number of topological interactions. The change in viscoelastic properties of micellar hydrogel after addition of STA was studied by rheological analysis. The studied triblock copolymer appears to be a suitable tissue engineering material as a carrier in applications for targeted drug delivery and tissue regeneration.
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