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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Analýza bakteriálních izolátů a DNA ze společenstva ze čtyř různých sukcesních ploch na území po těžbě hnědého uhlí
 Chroňáková, Alica ; Halbritter, A. ; Krištůfek, Václav ; Biró, B.
Microbial communities of 4 different stages (initial [0 years after heaping of the excavated material], early [10], middle [20] and late [42] of primary succession process were studied. They were characterized by genotyping methods: ARDRA - Amplified ribosomal DNA restriction analyses and box-PCR - genomic fingerprinting using specific primer for boxA-element. Box-PCR method was chosen for comparison of methodological approaches used for characterization of heterotrophic bacterial communities. ARDRA patterns of eubacterial communities were so complex and there weren´t shown any differences among individual communities on different stages of primary succession process. Actinomycetes communities were analyzed using two sets of primers: Actinomycetes 1. (243F-1378R, Heuer et al. 1997) and Actinomycetes 2. (243F-A3R; Monciardini et al. 2002). Fingerprints, which were obtained using both of actinomycetes specific primers, showed differences between communities on the early and later stages of primary succession process. Boy-PCR analysis is still in data processing and will be published later
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