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The chitosan-glucan complex isolated from Schizophyllum commune
Krčmář, Martin ; Obruča, Stanislav (referee) ; Omelková, Jiřina (advisor)
Chitosan-glucan complex is fungal origin copolymer that finds application in medicine and cosmetics. Traditionally mycelium of Aspergillus and Penicillium is considered as industrial chitosan-glucan complex source, though utilization of Micromycetes in biotechnological productions is sometimes undesirable. The aim of the work was to study the possibility of Basidiomycete Schizophyllum commune submerged cultivation for industrial scale chitosan-glucan complex production use. Within the work there was studied effect of cultivation conditions (type and concentration of carbon sources in nutrient medium, ratio of carbon source to nitrogen source, medium initial pH and aeration intensity) on Sch. commune #127 mycelium growth, chitosan-glucan complex formation and exopolysaccharide synthesis. As the result, the method for chitosan-glucan complex production increase and exopolysaccharide synthesis suppression was suggested. Chitosan-glucan complex from Sch. commune #127 submerged mycelium was separated by successive alkali and acid treatments. Effects of alkali concentration and application technique, and type of acid on physical and chemical properties of chitosan-glucan complex were described. Analytical methods for in process control and final product characteristics were suggested.
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The chitosan-glucan complex isolated from Schizophyllum commune
Krčmář, Martin ; Obruča, Stanislav (referee) ; Omelková, Jiřina (advisor)
Chitosan-glucan complex is fungal origin copolymer that finds application in medicine and cosmetics. Traditionally mycelium of Aspergillus and Penicillium is considered as industrial chitosan-glucan complex source, though utilization of Micromycetes in biotechnological productions is sometimes undesirable. The aim of the work was to study the possibility of Basidiomycete Schizophyllum commune submerged cultivation for industrial scale chitosan-glucan complex production use. Within the work there was studied effect of cultivation conditions (type and concentration of carbon sources in nutrient medium, ratio of carbon source to nitrogen source, medium initial pH and aeration intensity) on Sch. commune #127 mycelium growth, chitosan-glucan complex formation and exopolysaccharide synthesis. As the result, the method for chitosan-glucan complex production increase and exopolysaccharide synthesis suppression was suggested. Chitosan-glucan complex from Sch. commune #127 submerged mycelium was separated by successive alkali and acid treatments. Effects of alkali concentration and application technique, and type of acid on physical and chemical properties of chitosan-glucan complex were described. Analytical methods for in process control and final product characteristics were suggested.
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Optimalizacion submerged cultivation of select types enthomopathogenic fungi
SUCHANOVÁ, Michala
This graduation theses was intent on study influence conditions submerged cultivation select types enthomopatogenic fungi in liquid nutritive medium with emphasis on optimalization key elements of the process that manner performance uniform biomass mythosporotic fungi {--} blastospores. Experimental part of work was conceived with regard on next sphere problems: 1.Nutritive soil compositionon effect on production blastospores 2.Comparing possibility different kinds and strains enthomopathogenic fungi produce blastospores in submerged cultivation. 3.Conditions submerged cultivation effect on production and yield blastospores 4.Verify possibility production of uniform biomass blastospores in range usable for large-screen application
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