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Permeation of nanoparticles across sublingual membrane 4
Horejšová, Lenka ; Doležal, Pavel (advisor) ; Musilová, Marie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Consultant: doc. RNDr. Pavel Doležal, CSc. Student: Lenka Horejšová Title of Thesis: Permeation of Nanoparticles Across Sublingual Membrane 4 The theoretical part describes the anatomy and physiology of oral cavity and sublingual drug administration. It also describes theory of nanotechnology and the characterization of nanoparticles with a focus on nanoparticle drug forms and there are also mentioned methods and mesuring device. . The experimental part describes in vitro permeation experiment of nanoparticles (Nano beads based on PD, Chromeon 470 marked, carboxylated). Permeation of this nanoparticles through porcine sublingual membrane (1 cm2) . Thesis compares nanoparticles transfer of phosphate buffer pH 6.6 and citrate buffer pH 6.6 to the acceptor phase consisting of phosphate buffer pH 7,4. First permeation experiment works with frozen sublingual membrane, a second experiment with the native membrane. The dimension of nanoparticles and aggregates of nanoparticles in dispersions were measured before the start of permeation by the dynamic light scattering. This work demonstrates that the choice of donor buffer has not an important influence. Key words: nanoparticles, fluorescent...
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Permeation of nanoparticles across sublingual membrane 3
Šašurová, Michaela ; Doležal, Pavel (advisor) ; Vachek, Josef (referee)
4 ABSTRACT The theoretical part describes anatomical and physiological aspects of the oral cavity, tongue and salivary glands. It captures the issue of sublingual administration of drugs. Through a brief history of nanotechnology it comes to characterize nanoparticles as basic building blocks of nanostructures. It describes the production and properties of nanoparticles, shows the advantages of using nanoparticles as a drug delivery system, but also point out their potential toxicity. At the end of the theoretical part principles and methods of measurement used in the experiments are summarized and characterized. In the experimental part in vitro permeation experiments are described and evaluated. Permeation of nanoparticles marked Chromeon 470 through porcine sublingual membrane in area of 1 cm2 from the donor phase of pH 6.6 to acceptor phase pH 7.4 was observed in these experiments. For the permeation experiments were used both fresh and long-term frozen sublingual membranes fixed in Franz diffusion cells with glue or silicone urease. Before starting the permeation experiments the size of nanoparticles or agglomerates were characterized using dynamic light scattering and by fluorescence microscopy. It was verified that the two evaluated methods of fixing of membranes in diffusion cells have no...
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Permeation of nanoparticles across sublingual membrane 4
Horejšová, Lenka ; Doležal, Pavel (advisor) ; Musilová, Marie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Consultant: doc. RNDr. Pavel Doležal, CSc. Student: Lenka Horejšová Title of Thesis: Permeation of Nanoparticles Across Sublingual Membrane 4 The theoretical part describes the anatomy and physiology of oral cavity and sublingual drug administration. It also describes theory of nanotechnology and the characterization of nanoparticles with a focus on nanoparticle drug forms and there are also mentioned methods and mesuring device. . The experimental part describes in vitro permeation experiment of nanoparticles (Nano beads based on PD, Chromeon 470 marked, carboxylated). Permeation of this nanoparticles through porcine sublingual membrane (1 cm2) . Thesis compares nanoparticles transfer of phosphate buffer pH 6.6 and citrate buffer pH 6.6 to the acceptor phase consisting of phosphate buffer pH 7,4. First permeation experiment works with frozen sublingual membrane, a second experiment with the native membrane. The dimension of nanoparticles and aggregates of nanoparticles in dispersions were measured before the start of permeation by the dynamic light scattering. This work demonstrates that the choice of donor buffer has not an important influence. Key words: nanoparticles, fluorescent...
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