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Study of potential anti-adherence compounds in a lung cell model as a means for the prophylaxis of bacterial infections in cystic fibrosis
Áčová, Andrea ; Hodek, Petr (advisor) ; Nosková, Libuše (referee)
5 Abstract Cystic fibrosis (CF) is an insidious genetic disease with autosomal-recessive inheritance. The impaired function of the CFTR protein, caused by a mutation in the eponymous gene, results in a wide range of symptoms, the most serious being the effect on the respiratory system. The main impact on the respiratory system is the appearance of thick mucus, which contributes to the accumulation of bacterial cells in the patient's lungs. Life-threatening pathogens include Burkholderia cenocepacia (BC) and Pseudomonas aeruginosa (PA). These bacteria produce several virulence factors such as BC2L-A or BC2L-C lectins in BC and PA-IIL in PA. The virulence factors allow bacteria to bind via protein-saccharide interactions to lung cells with altered glycosylation, which is seen in the CF patient. This diploma thesis focuses on the above-mentioned lectin interaction and the inhibitory effect of hen IgY antibodies or trivalent fucosylated glycoclusters on the adherence of BC (strain ST-32, CCM 7291) / PA (strain PAK, ST 1763) to lung epithelial cells of the CuFi-1 (from a CF patient) / NuLi-1 (from a healthy donor). The possible influence of glycoclusters on PA (PA-lux strain) cell viability was studied, but no significant effect was observed. To verify the expression of the BC2L-C lectin, it was necessary to...
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