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Regulation of phosphate uptake by strigolactones
Balín, Michal ; Vaňková, Radomíra (advisor) ; Tylová, Edita (referee)
This Bachelor thesis is focused on the reactions of plants to low levels of available phosphorus, which is essential element for plant nutrition. In the context of plant stress, plant hormones strigolactones play very important role. Strigolactones influence growth and development of the whole plant. Biosynthesis of strigolactones increases under phosphate deficiency. This thesis consists of three parts. The first part is concentrated on phosphorus, its compounds and its availability in the environment. The second part is mainly dedicated to phosphate stress - its sensing by plants, their adaptations to stress and local as well as long distance signalling. Attention is also paid to phytohormones, namely auxins, cytokinins, gibberellins, ethylene, abscisic acid and other signal molecules, like sucrose, the phosphate molecule itself and microRNAs. The third part is focused mainly on the structure, biosynthesis, signal transduction and the mode of action of strigolactones during phosphate stress. Keywords: phosphate, deficiency, strigolactone, phytohormones, stress
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Vliv strigolaktonu na větvení kořenů u rostlin huseníčku rolního
Macoszek, Ondřej
In this bachelor study the influence of the concentration of GR24, the synthetic analogue of strigolactone, on the length of the primary root of different ecotypes of Arabidopsis plants was studied. Six ecotypes of A. thaliana were chosen and the experiment was performed at the Gregor Mendel Institute in Vienna in the laboratory of Dr. Wolfgang Busch. The used GR24 concentrations ranged from 12.5 nM to 50 microM. It was known from previous work (Ruyter and Spira 2010, Kol-tai 2011, Vacková 2013) that GR24 influences the root architecture of Arabidopsis thaliana, but the results differed. From the experimental results it is clear that the influence of GR24 on the primary root length depends on the individual plant genotype, but generally it is possible to suggest that low strigolactone concentrations cause inhibition, subsequently higher stimulation of the primary root length and finally concentration above 10 microM are highly inhibitory in all genotypes. Based on the results the concentration of 100 nM was chosen for the subsequent Genome wide association study (GWAS).
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