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Phytotoxicity of selected naphthoquinones on a selected plant model
Rucký, Jakub ; Masařík,, Michal (referee) ; Provazník, Ivo (advisor)
The introductory part of this thesis is focused on the theoretical analysis of solved problems as examining the toxicity of naphthoquinones plumbagine and juglone, especially with regard to their allelopatic action. The next section is focuses on the plant stress, caused by the action of stress factors leading to their death. There is an experimental protocol and the possibilities of determination the effect of naphthoquinones on the plant model. Experimental section discusses changing growth parameters of the plant samples in different concentrations of the naphthoquinone. There is examined cell viability and changes in the synthesis of the secondary metabolites. Data obtained by using spectrophotometric and microscopic analysis are evaluated with STATISTICA software and statistical significance are plotted.
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Dissecting the effects of salicylic acid on redox balance in plant cells using biochemical and fluorescent imaging techniques
Růžičková, Gabriela ; Burketová, Lenka (advisor) ; Janda, Martin (referee)
Topic of bachelor thesis is plant immunity, specifically it focuses on salicylic acid, reactive oxygen species, it also analyses hox salicylic acid effects formation of reactive oxygen species and which proteins are involved in this biosynthesis. Also in this thesis is described basic methods for measuring of reactive oxygen species, what are advantages, disadvantages, how they work and what they can be used for. Salicylic acid is one of phytohormones involved in immune reaction in plant defence, this thesis is describing salicylic acid signalling, associated proteins and overall effect on plant. Reactive oxygen species have wide framework of action in organisms, they can be called double-edged sword, they can help plant, but also, they can harm depending on their concentration and regulation of their forming, the problem of their quenching is also described in this thesis - their formation, antioxidants, effect on plant immunity. Chapter about measuring methods is divide into three parts - fluorescence methods, chemiluminescence methods and spectrophotometric (histochemical) methods. Key words: salicylic acid, reactive oxygen species, plant immunity, plant stress, phytohormones
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Metabolom rostlin a jeho proměny v reakci na stres
Dobešová, Markéta
Adverse environmental effects have a significant impact on plants' life and influ-ence the plants' metabolome. Specific stress-related responses are manifested both on plant development and its metabolites. This thesis entitled "Plant metabolome and its response to stress" outlines the composition of plant metabolome and reviews stress-related metabolites and metabolomics processes. The experimental part summarizes the results of two independent experiments. First, Hordeum vulgare response to stress was followed via targeted LC-MS metabolomics and proteome analysis. Next, a drought response was analyzed in Solanum lycopersicum via GC-MS metabolome profiling. Results highlighted a tissue-specific response to stress and pinpointed several putative abiotic stress markers
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Perspektivní hnojiva pro produkci okrasných dřevin v kontejnerech
Plchová, Andrea
The task of this work was to evaluate the prospective use of fertilizers in containerproduction and their impact on ornamental trees. The experiment was established in April 2011 on the land department the Faculty of Horticulture in Lednice in one growing season. It was used five variants of substrates. For variant I was used peat-bark substrate (RKS II), which was regularly fertilizing with Kristalon fertilizer. For variant II was used substrate RS II with Osmocote. For the other three variants was used substrate RS II with low content (var. III), medium content (var. IV) and high content of organic fertilizers (var. V). Experimental plant was Philadelphus x hybr. 'Mont Blanc'. During the growing season was measured the plant height, at the end of the vegetation was measured strength of the root neck, root volume and number of stems. The variant II with Osmocote achieved the best results, the control variant I reached the second best results. The variants III, IV and V with organic fertilizers fell the worst, the variant III with low content of organic fertilizers fell the worst of all.
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Možnosti využitia antistresových prípravkov pri pestovaní zeleninovej sadby
Jasenovcová, Lenka
The aim of this thesis was to develop an overview on the utilization anistress preparation and use on selected plant vegetable seedlings cabbage (Brassica oleracea) variety 'Pluto', yellow peppers (Capsicum annuum) L. (partim) variety 'Zlata', tomato (Solanum lycopersicum L.) variety 'Idyll' and lettuce (Lactuca sativa L. var. capitata variety 'Lednický' to improve their resistance. For the experiment were chosen preparations Agrisorb, Atonik Pro, Lexin, Synergin and Pentakeep. Experimental part is focused the results of which are evaluated morphological parameters of the seedlings trial which took place in 2015 at the Faculty of Horticulture Mendel University in Lednice.
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Možnosti využití antistresových přípravků při pěstování zeleninové sadby
Balcar, Miroslav
Literature research seeks to approach the ecological and biodynamic agriculture, external stressors and anti-stress products in plants. Describing the technology of pre-cultivated vegetable seedlings and species. The practical part is devoted to monitoring the environmental effects of plant anti-stress experiment based on the plots of the Horticultural Faculty in Lednice na Moravě. An attempt was devoted to impact on growth plants after the application of selected products Agrisorb, FERBIFLOR?, EM AKTIV, Kopřiva Plus and Roháček (BD 500). Statistical analysis showed effects on the individual morphological characteristics of plants.
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Phytotoxicity of selected naphthoquinones on a selected plant model
Rucký, Jakub ; Masařík,, Michal (referee) ; Provazník, Ivo (advisor)
The introductory part of this thesis is focused on the theoretical analysis of solved problems as examining the toxicity of naphthoquinones plumbagine and juglone, especially with regard to their allelopatic action. The next section is focuses on the plant stress, caused by the action of stress factors leading to their death. There is an experimental protocol and the possibilities of determination the effect of naphthoquinones on the plant model. Experimental section discusses changing growth parameters of the plant samples in different concentrations of the naphthoquinone. There is examined cell viability and changes in the synthesis of the secondary metabolites. Data obtained by using spectrophotometric and microscopic analysis are evaluated with STATISTICA software and statistical significance are plotted.
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Možnosti využití antistresových přípravků při pěstování zeleninové sadby
Sijková, Adriana
The teoretical part of the thesis seeks of the zoom and description of plant stress, the possibility of using anti-stress products and their effects on plants. Description of selected vegetable species and the impact date for sowing seed. Practical work is dedicated to monitoring anti-stress effect products, their effects on plants and the effect on the overall structure of plants. After statistical analysis has been evidence for an effect of the individual plant morphological characteristics of plants.
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Comparison of seasonal changes of ecophysiological characteristic of two selected trees under stress of the urban environment
ŠINDELÁŘOVÁ, Lenka
This thesis deals with the study of impact of stress to the two types of broadleaved trees in urban environment during the growing season. The studied species were Small-leaved Lime (Tilia cordata) and London Planetree (Platanus acerifolia). Trees are the important part of the urban environment both from an aesthetic standpoint and from the standpoint of the reduction of negative effects of the urban environment. However, these trees are under the constant influence of stress factors with which they have to deal (air pollution, water, temperature). Some trees deal with stress better but some worse. Because of this fact, two different types of trees were selected, which are able to deal with stress by their own way. As indicators of physiological state of plants under stress, the content of photosynthetic pigments and photochemical efficiency of PS II (QY and QY') were monitored at individual trees. The measurements were carried out always two days consecutively from May to September 2013 in Jiráskovo nábřeží in České Budějovice. Every month, the spectrophotometric measurement to determine the pigments in a leaf was carried out. For the spectrophotometric measurements, twenty-four leaves were measured in total; for the fluorescent measurement, thirty leaves were measured in total. The obtained values of both methods were processed and evaluated by graphs, which together with the average values show the statistic demonstrability of difference between the measured trees in city. According to these measured values, Small-leaved Lime seemed to be more vital at a given location, although London Planetree is marked in the literature as more resistant one towards the urban stress.
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