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Zavedení a optimalizace metodiky pro stanovení lipofilních a hydrofilních vitamínů v biologických vzorcích
Kalábová, Vendula
The main scope of this diploma thesis are the methods used for building vitamins, especially priorizing chromatographic methods. You can also find the characteristics of vitamins dissolvable in fat and water, their physiological meaning, daily use, presence in foodstuffs and methods, which are suitable for their analysis. The experimental part describes the sample preparation, their vitamin extraction methods, the requierements for chromatographic assessions, methods of qualitative and quantitative evaluation of chromatographs with the focus on the calibration curve method. The work also puts an emphasis on establishing the limit of detection and the limit of quatification, and determining the lipophilic vitamins in matrices rich of these vitamins using the method of HPLC.
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Charakteristika a metody stanovení vitaminů v masozeleninových přesnídávkách určených pro dětskou výživu
Piechowiczová, Markéta
Bachelor thesis Characteristics and methods for determination of vitamins in meat-vegetables purées intended for baby food was focused on acquiring general knowledge about child nutrition, with regard to adequate intake of vitamins in the diet, which are then characterized, together with methods of their determination. In the first part there are described particular periods of nourishment until 12th month of life. Nourishment recommendations are summarized here, more specifically required nutrients in this age, but also issues concerning allergies, alternative nourishment and some diseases, which can lead to hypovitaminosis. The second part is devoted to the characteristics of vitamins. Chemical properties, effect on the body, occurrence in foods and the recommended daily intake are included. The chapter about occurrence in food is always accompanied by the recommendation of ingredients with a high content of vitamins, which are appropriate to add to complementary food. In the last part of thesis the methodology for determination of vitamins are summarized in the light of the newest trends. These methods include chromatographic methods, spectrophotometric,fluorimetric, polarographic, microbiological and capillary electrophoresis.
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