|
|
Molecular detection of intestinal parasites in laboratory rodents
FÜRSTOVÁ, Petra
The master's thesis focuses on the issue of molecular detection by real-time PCR in laboratory rodents using primers for TaqMan real-time PCR. The designed primers were verified on 22 samples of buccal swabs, biopsies from intestinal tissue, and excrement. Specificity was verified on 9 related intestinal parasites. The detection limits of the reaction mixtures were also determined. The detection limit of the reaction mixture for Spironucleus muris was set at 3.3×10-5 ng/l, for Tritrichomonas muris at 9.4×10-8 ng/l and for Giardia muris at 1.66×10-6 ng/l. Many disease agents exist among laboratory rodents. The presence of these agents needs to be detected. Molecular detection is one of the most sensitive detection methods. Nowadays, only a few designed essays are available for detection of intestinal parasites, which include Spironucleus muris, Tritrichomonas muris and, last but not least, Giardia muris. The necessity to detect these parasites has increased due to the demand for SPF (specified-pathogen free) laboratory rodents for experimental purposes. The intestinal parasites are often used as an indicator of the failure of micro-isolation technology. Also, intestinal parasites can cause very unpleasant diseases that need to be treated. The aims has been met. The optimized methods are suitable for use in the detection of Spironucleus muris, Tritrichomonas muris and Giardia muris. The results of the thesis can serve as an information source and can be used for other investigative purposes.
|
|
|
Střevní paraziti ptáků na Svalbardu
ŽÁNOVÁ, Veronika
The study aimed to obtain essential knowledge about the distribution of intestinal parasites of birds occurring in Svalbard. Noninvasive coprological techniques were used to detect coccidian, cryptosporidian and microspordian parasites. The research included field collection of faecal samples and laboratory examination by microscopic and molecular methods.
|
|
|
Possible effect of patogens on fttness of selected duck species
Langrová, Anna ; Votýpka, Jan (referee) ; Musil, Petr (advisor)
In total, 76 birds, mainly breeding females, were captured in years 2007-2009. Birds were inspected for the presence of avian influenza (AI) viruses and the AI antibodies. They were inspected as well for the presence of bacteria Borrelia burgdorferi s. l. and Anaplasma phagocytophilum and protozoan Leucocytozoon simondi in blood. The feces were examined for the oocysts and eggs of intestinal parasites. AI viruses were found in 4 cases, while over 80% of inspected birds had AI antibodies. Bacteria B. burgdorferi s. l. and A. phagocytophilum were found in 2 and 1 case respectively. Protozoan L. simondi was not found in any blood smear. The oocysts from protozoan genera Eimeria and Tyzzeria and eggs from roundworm genera Capillaria, Amidostomum and Echinuria were observed in feces. Statistical analyses were due to the small size of our data set inconclusive.
|
|
|
The role of a nurse in a prevention and a treatment of intestinal parasites of children
JANDOVÁ, Anna
The best-known intestinal parasites are Pinworms, Roundworms, Tapeworms and the disease called Toxocarosis. The oftenest parasite is, according to sources, the Pinworm. Intestinal parasites affect mostly young children of pre-school age, sometimes even older. The first goal of the thesis was to investigate parents' awareness of the prevention of parasitic diseases in children. There is a hypothesis set to this goal: Parents of children who suffered from parasitic disease are more informed than parents of children who have not had this disease. The second goal was to map the specifics of nursing care for children with parasitic diseases at GPCHA practice. A research question was chosen for this aim: What are the specifics of nursing care at GPCHA in parasitic diseases? In methodology, the empirical part was chosen and it was a qualitative - quantitative research survey. For the quantitative part, the interrogation method and technique of non-standardized questionnaire was used. The research sample of quantitative survey consisted of 223 respondents, it means parents whose child is aged from 0 to 6 years. The questionnaires were distributed on social networking and others in a nursery school in Týn nad Vltavou. At the very beginning, respondents were acquainted with the topic of the thesis. The results of a quantitative survey were processed with the help of a data matrix and were further processed within twenty-arranged bar charts. To verify the hypothesis, we used the Chi- Quadrat test, which in this case is not satisfactory, therefore, there had to be chosen to merge the responses. An interrogation method, technique-depth interview was used in the qualitative part. The research group consisted of five nurses; three nurses working at GPCHA in Týn nad Vltavou and two working at GPCHA in České Budějovice. While processing the interviews, there open coding was used and an analysis of interviews was conducted by using the method of Pencil and Paper Interview. The results of this bachelor thesis will be published in the journal Paediatrics practice.
|
|
|
Střevní paraziti ze středověké lokality v Praze
ŘÍHOVÁ, Tereza
The aim of this thesis was to examine the archeological material from medieval site in Prague and identify human or animal intestinal parasite eggs or cysts. The concetration methods were used for parasite identification and polymerase chain reaction (PCR) was used for detection of parasitic protist DNA. In comparision with the results of most of similar research, the occurrence of parasites was low.
|
|
|
Intestinal parasites in archeological material from medieval period
BRUZLOVÁ, Pavlína
The aim of this thesis was to search for and identify human and/or animal intestinal parasite eggs in archeological material from medieval period in Brno and Prague. Concentration methods - sedimentation and flotation were used for parasite identification. Polymerase chain reaction (PCR) was used for the detection of parasite DNA. The results were put into context with other data published in similar studies and research.
|
|
|
Intestinal parasites of vertebrates in Svalbard
MYŠKOVÁ, Eva
The study was aimed to obtain basic information about the distribution of intestinal parasites of mammals and birds in Svalbard. Faeces of different species were used to detect intestinal parasites. All samples were collected during two seasons and examined by microscopic methods and molecular diagnostic was used for detection of microsporidia, cryptosporidia, coccidia and giardiae.
|
|
| |
guest ::
