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Voltammetric Determination of 5-Nitrobenzimidazole Using Non-Traditional Electrode Materials
Chládková, Barbora ; Vyskočil, Vlastimil (advisor) ; Zima, Jiří (referee)
5 Abstract This Diploma Thesis is targeted on the determination of 5-nitrobenzimidazole (5-NBIA) using following techniques: DC voltammetry (DCV) and differential pulse voltammetry (DPV). As working electrodes, a silver amalgam paste electrode (AgA-PE), which was constructed for this determination in the form of a new prototype, a bismuth film electrode (BiFE), at which the optimum conditions for the deposition of the bismuth film at a suitable substrate (glassy carbon electrode and gold electrode were tested for this purpose) were initially optimized, and a glassy carbon electrode (GCE) were used. The optimum conditions for the voltammetric determination of 5-NBIA at the AgA-PE (in a medium of Britton-Robinson buffer (BR-buffer) of pH 7.0 for both DCV and DPV), at the BiFE with gold substrate (BR-buffer of pH 9.0 for both DCV and DPV; the film was deposited "ex situ" in a stirred plating solution (1000 mg L-1 Bi(III) solution in 0.1 mol L-1 acetate buffer of pH 4.5) for 300 s), and at the GCE (BR-buffer of pH 5,0 for both DCV and DPV). Under these conditions, calibration dependences were measured in the concentration ranges of 0.1 - 100 µmol L-1 (pro DCV a DPV na AgA-PE) and 1 - 100 µmol L-1 (pro DCV a DPV na BiFE a GCE), and the limits of quantification (LQs) were calculated for particular methods: LQ ≈...
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Voltammetric Determination of 5-Nitrobenzimidazole Using Non-Traditional Electrode Materials
Chládková, Barbora ; Vyskočil, Vlastimil (advisor) ; Zima, Jiří (referee)
5 Abstract This Diploma Thesis is targeted on the determination of 5-nitrobenzimidazole (5-NBIA) using following techniques: DC voltammetry (DCV) and differential pulse voltammetry (DPV). As working electrodes, a silver amalgam paste electrode (AgA-PE), which was constructed for this determination in the form of a new prototype, a bismuth film electrode (BiFE), at which the optimum conditions for the deposition of the bismuth film at a suitable substrate (glassy carbon electrode and gold electrode were tested for this purpose) were initially optimized, and a glassy carbon electrode (GCE) were used. The optimum conditions for the voltammetric determination of 5-NBIA at the AgA-PE (in a medium of Britton-Robinson buffer (BR-buffer) of pH 7.0 for both DCV and DPV), at the BiFE with gold substrate (BR-buffer of pH 9.0 for both DCV and DPV; the film was deposited "ex situ" in a stirred plating solution (1000 mg L-1 Bi(III) solution in 0.1 mol L-1 acetate buffer of pH 4.5) for 300 s), and at the GCE (BR-buffer of pH 5,0 for both DCV and DPV). Under these conditions, calibration dependences were measured in the concentration ranges of 0.1 - 100 µmol L-1 (pro DCV a DPV na AgA-PE) and 1 - 100 µmol L-1 (pro DCV a DPV na BiFE a GCE), and the limits of quantification (LQs) were calculated for particular methods: LQ ≈...
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Voltammetric Determination of 5-Nitrobenzimidazole at a Silver Amalgam Paste Electrode
Chládková, Barbora ; Vyskočil, Vlastimil (advisor) ; Zima, Jiří (referee)
This bachelor's thesis concerns the determination of 5-nitrobenzimidazole (5-NBIA) by the methods DC voltammetry (DCV) and differential pulse voltammetry (DPV). A silver amalgam paste electrode (AgA-PE), which was prepared for this determination in the laboratory, was used as a working electrode. The optimum conditions for voltammetric determination of 5-NBIA were found and under these conditions, concentration dependences were measured and the limits of quantification (LQ) were calculated for each method. The media of BR-buffer pH 7.0 and pH 5.0 were chosen as optimum for DCV determination of 5-NBIA at AgA-PE and DPV determination of 5-NBIA at AgA-PE, respectively. For both DCV and DPV, the linear concentration dependences were obtained in concentration ranges of 5-NBIA from 2 × 10-7 mol/l to 1 × 10-4 mol/l. The attained LQ were found to be 6 × 10-7 mol/l (for DCV at AgA-PE) and 2 × 10-7 mol/l (for DPV at AgA-PE).
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