Original title: Voltametrické stanovení 5-nitrobenzimidazolu na stříbrné amalgamové pastové elektrodě
Translated title: Voltammetric Determination of 5-Nitrobenzimidazole at a Silver Amalgam Paste Electrode
Authors: Chládková, Barbora ; Vyskočil, Vlastimil (advisor) ; Zima, Jiří (referee)
Document type: Bachelor's theses
Year: 2010
Language: cze
Abstract: [cze] [eng]
Tato bakalářská práce se zabývá stanovením 5-nitrobenzimidazolu (5-NBIA) metodami DC voltametrií (DCV) a diferenční pulsní voltametrií (DPV). Jako pracovní elektroda byla použita stříbrná amalgamová pastová elektroda (AgA-PE), která byla pro toto stanovení připravena přímo v laboratoři. Byly nalezeny optimální podmínky pro voltametrické stanovení 5-NBIA a za těchto podmínek proměřeny kalibrační závislosti a vypočteny meze stanovitelnosti (LQ) pro jednotlivé metody. Jako optimální prostředí pro DCV stanovení 5-NBIA na AgA-PE bylo zvoleno prostředí BR-pufru o pH 7,0, pro DPV stanovení BR-pufru o pH 5,0. Pro obě voltametrické metody byly získány lineární koncentrační závislosti proudu píku 5-NBIA v rozmezí 2 . 10-7 mol/l až 1 . 10-4 mol/l. Pro metodu DCV na AgA-PE bylo dosaženo LQ ~ 6 . 10-7 mol/l a pro metodu DPV na AgA-PE LQ ~ 2 . 10-7 mol/l. This bachelor's thesis concerns the determination of 5-nitrobenzimidazole (5-NBIA) by the methods DC voltammetry (DCV) and differential pulse voltammetry (DPV). A silver amalgam paste electrode (AgA-PE), which was prepared for this determination in the laboratory, was used as a working electrode. The optimum conditions for voltammetric determination of 5-NBIA were found and under these conditions, concentration dependences were measured and the limits of quantification (LQ) were calculated for each method. The media of BR-buffer pH 7.0 and pH 5.0 were chosen as optimum for DCV determination of 5-NBIA at AgA-PE and DPV determination of 5-NBIA at AgA-PE, respectively. For both DCV and DPV, the linear concentration dependences were obtained in concentration ranges of 5-NBIA from 2 × 10-7 mol/l to 1 × 10-4 mol/l. The attained LQ were found to be 6 × 10-7 mol/l (for DCV at AgA-PE) and 2 × 10-7 mol/l (for DPV at AgA-PE).
Keywords: 5-Nitrobenzimidazole; DC Voltammetry; Diferential Pulse Voltammetry; Silver Amalgam Paste Electrode; Voltammetry; 5-nitrobenzimidazol; DC voltametrie; diferenční pulsní voltametrie; stříbrná amalgamová pastová elektroda; voltametrie

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/39331

Permalink: http://www.nusl.cz/ntk/nusl-303582


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Bachelor's theses