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Use of chicken antibodies against PAIIL lectin for prevention of Pseudomonas aeruginosa infections in patients with cystic fibrosis
Kubíčková, Božena ; Hodek, Petr (advisor) ; Anzenbacherová, Eva (referee) ; Eckschlager, Tomáš (referee)
Cystic fibrosis (CF) is one of the relatively common inherited diseases caused by a mutation in the gene encoding for CFTR protein, which forms a chloride channel that significantly affects ion homeostasis and the associated fluid management of the cell. This disease mainly affects the respiratory and digestive systems, being the most life-threatening in the respiratory tract. Patients with CF suffer from frequent and recurrent respiratory infections that lead to the development of chronic inflammation and gradual destruction of lung tissue. These lung infections, which are caused mostly by the opportunistic pathogen Pseudomonas aeruginosa, are the most common cause of morbidity and mortality in these patients. At present, antibiotics are used in the treatment of Pseudomonas aeruginosa infections, but new methods of antibacterial therapy need to be found to overcome the development of resistance. In addition to active immunization of CF patients against Pseudomonas aeruginosa, their passive immunization with specific chicken antibodies directed against this pathogen offers promising possibilities. This dissertation thesis is aimed to verify the prophylactic potential of hen IgY antibodies against the virulence factor Pseudomonas aeruginosa - lectin PAIIL, and to further develop an experimental...
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Chicken antibodies - tool for passive immunization of Cystic fibrosis patients
Hadrabová, Jana ; Hodek, Petr (advisor) ; Kubíčková, Božena (referee)
Cystic fibrosis (CF) is a congenital disease which affects respiratory and gastrointestinal system. It is the autosomal recessive disorder and it arises from the CFTR (cystic fibrosis transmembrane conductance regulator) gene mutation. The CFTR is an ion channel for chloride ions transmission. If its function is limited, the pulmonary mucus becomes dense and microorganisms are able to infect the lungs and grow effectively. Most common and dangerous bacteria in CF patients lungs is Pseudomonas aeruginosa. Antibiotics are used as the first therapy, but the bacteria becomes resistant against them. The chronic inflammation arises and it proceeds to the pulmonary tissue destruction (fibrosis). New methods of CF patients therapy are searched especially because of antibiotics resistance. The promising approach could be the passive immunization with chicken antibodies specific against Pseudomonas aeruginosa virulence factors. The IgY solution is suitable to be converted to aerosol by nebuliser and the patient could inhale that. As the IgY immunoglobuline can be an inflammatory substance when inhaled, it should be tested what causes in healthy lungs. The Wistar rat was used as a suitable experimental model. At first the chicken antibodies were isolated, then the Fab fragment was prepared by digestion. The...
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