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Detekce polymorfismu genu CYB5A ve vztahu ke skatolu, indolu a androstenonu u kanců
Lávičková, Monika
The presence of boar taint has a negative impact on the meat quality and it is prominent even after the cooking. It is caused mainly by the high concentration of phero-mones androstenone, indole and skatole present in fat tissues of animals. This bachelor thesis studies the problems of boar taint in uncastrated males and its impact on the quality of meat. Mainly the polymorphism of the gene CYB5A and its correlation with and-rostenone, indole and skatole was studied. In this thesis, the PCR-RFLP method was used to determine the genotypes of 40 male samples. Subsequently, the frequencies of alleles and genotypes were calculated. Using the SAS software, resulting data were statistically evaluated by association rules in studied genotypes CC and CT in relation to observed traits. The p-values of all samples were higher than 0,05 and thus the association of observed traits with the C>T polymorphism was not conclusive.
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Variabilita genu HSD3B ve vztahu k hladině androstenonu a skatolu ve hřbetním tuku kanců
Novotná, Pavla
The bachelor thesis describes the reasons for the origin of wild boar taint, metabolism of the substances causing a typical odour as well as the possibility of its removal. Ways to eliminate or suppress a boar odour include surgical castration of boars, immunocastration, nutrition and breeding techniques. Nowadays, the genetic influence and possible polymorphisms influencing the unpleasant odour are being studied. Specifically, the variability of the HSD3B gene in relation to the level of androstenone and skatole in the dorsal fat of boars is specifically described.
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The effect of live weight on androsterone and skatole content in adipose tissue of boars
Poláčková, Miroslava ; Okrouhlá, Monika (advisor) ; Michaela, Michaela (referee)
The aim of this study was to provide a comprehensive literature review and evaluation of research on the influence of androstenone and skatole for boar live weight, which thanks to modern trends sensitive issue.
Skatole is malodorous indole compound, which is formed in the colon of pigs later leads to deposition in adipose tissue, causing the so-called. Boar odor.
Steroid androstenone is produced by Leydig cells of the testes boars, when a part is floated urine, partly accumulate in saliva, to stimulate the sows and part is accumulated in the adipose tissue.
The chromatographic method suitable for detecting substances responsible for boar odor was developed on a gas chromatograph mass spectrometer using chemical standards.
According to the results, we can evaluate that the differences between imunocastraties and boars are minimal, not only in fattening values, but also at the height of the back fat or muscle growth musculus longissimus lumborum et thoracis. Only with differing indicators are the amount of androstenone and skatole, which confirms that feeding into higher slaughter weight pigs is unprofitable, because the amount of these substances is many times higher than allowed by European standard. The meat of boars is therefore inconvenient and are a much better choice imunokastráti.
The hypothesis that a live weight of influence on behalf of androstenone and skatole in fat tissue boars are so confirmed.
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