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Úprava kvality bioplynu
Paroulková, Petra
This diploma thesis follows to the bachelor thesis Utilization of Algae in Biogas Trans-formations, which was rather a research type and dealt with the removal of carbon diox-ide by photosynthesis of algae from biogas. The aim of this thesis is to examine the issue of undesirable substances in biogas, mainly hydrogen sulfide, and to verify the possibil-ity of using Chlorella pyrenoidosa Chick (IPPAS C2) for the biogas treatment. This ver-ification is based on scientific studies and hypotheses. This culture was grown in the proposed laboratory system in BG11 medium and in BG11 medium with reduced SO4-2 sulfate salts for eight days and nine days. Based on the results of the culture parameters, sulphate formation in the nutrient medium has been proven to be dependent on pH and oxygen. During the measurement, the decrease of hydrogen sulfide concentration was recorded after application of the given culture apparatus. The loss of hydrogen sulfide occurred due to absorption into the nutrient medium. The hypothesis of the ability of algae to consume the resulting sulfate from the medium for their own use has not been confirmed.
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The metabolism and signaling of hydrogen sulfide: the role of CBS-related proteins in Caenorhabditis elegans
Vozdek, Roman ; Kožich, Viktor (advisor) ; Macůrková, Marie (referee) ; Jiráček, Jiří (referee)
Hydrogen sulfide (H2S) is a toxic gas that causes respiratory failure and death at high concentrations, but at low concentrations, it functions as a signaling molecule in vasodilation and neuromodulation, and it protects cells and tissues from reperfusion injury, hypoxia, hyperglycemia and endothelial dysfunction. Several model organisms have been used to study the physiological roles and signaling pathways of H2S. The roundworm Caenorhabditis elegans is a remarkable model for studying the physiology, developmental biology and signaling of H2S; however, the metabolism of H2S in this animal is largely unknown. Cystathionine beta-synthase (CBS) is one of three H2S-producing enzymes in mammals. Notably, C. elegans possesses 6 genes that encode proteins homologous to CBS, namely cbs- 1, cbs-2, cysl-1, cysl-2, cysl-3 and cysl-4. In this thesis we studied the roles of these genes in H2S metabolism and signaling. First, we identified cbs-1 as the gene encoding CBS in C. elegans; the recombinant purified CBS-1 protein exhibited canonical CBS activity, and RNA interference-mediated silencing of cbs-1 resulted in decreased CBS activity and increased homocysteine levels in worm extracts, recapitulating the phenotypes of CBS deficiency in mammals. Notably, the nematode and human enzymes differ in their domain...
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