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Strukturní studie serpinu Iripin-5 z klíšťat \kur{Ixodes ricinus}
SPĚVÁKOVÁ, Nikola
The aim of this bachelor thesis was a crystallization of serpin Iripin-5 from ticks Ixodes ricinus. Iripin-5 is the most produced serpin in the salivary glands of ticks. The focus of this thesis was to prepare protein Iripin-5 and perform crystallization experiments. Obtained crystals of Iripin-5 were in diffraction quality.
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Příprava mutantního serpinu z klíštěte \kur{Ixodes ricinus}
EDEROVÁ, Monika
Point mutation altering arginin for tryptophan amino acid residue in P1 site of tick salivary serpin Iripin-1 was created using specific primers. Recombinant protein with this mutation in nucleotide sequence was then expressed in chemically competent Escherichia coli cells, extracted from them and purified by affinity and size-exclusion chromatography. To see the impact of the mutation on inhibitory function of Iripin-1, its ability to bind trypsin and form covalent complexes was evaluated.
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Anti-hemostatické účinky serpinů z klíštěte \kur{Ixodes ricinus}
EDEROVÁ, Monika
Antihemostatic effects of four recombinantly produced serpins of the tick Ixodes ricinus (IRS 2, IRS 3, IRS 5 and IRS 8) were tested using a method of flow cytometry. Fluorescently labeled antibodies against platelet surface molecules were used to determine the difference in platelet aggregation and activation after incubation of samples with specific serpin. To see how serpins affect coagulation pathway, the prothrombin time, activated partial thromboplastin time and thrombin time tests were performed.
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