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The role of IL-17 in kidney transplantation
Menšíková, Markéta ; Stříž, Ilja (advisor) ; Krulová, Magdaléna (referee)
The role of IL-17 in kidney transplantation - abstract Naive CD4+ T-lymphocytes (Thp) can develop into Th17 line in the presence of TGF- and IL-6. Th17 cells are characterized by expression of Ror- t and by production of interleukin-17 (IL-17). It is secreted as a glycoprotein homodimer. Binding to IL-17 receptor (IL-17R), which is present in all cell types, stimulates the production of proinflammatory cytokines and chemokines. The ratio of Th17: Treg in the graft showing signs of rejection is higher than in the graft without rejection. The presence of IL-17 in a culture of proximal tubular epithelial cells (PTEC) stimulates the production of IL-6, IL-8, MCP-1 and C3 complement component. Simultaneous action of IL-17 and CD40L synergistically increases the production of IL-6, IL-8 and RANTES. Signaling from the receptor on the surface of PTEC associated with its increased expression is effected via the src kinase and MAP kinase, and probably leads to the transcription factor NF- B. In rat models of transplantation, the IL-17 appears in allografts on the second day after surgery, the level rises until the fifth day, then decreases and disappears before the death of the animal. IL-17 is not detectable in isografts and negative controls. It appears before the IFN- , which had been considered a trigger of...
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Renal pathology and diagnostic methods
Špiková, Andrea ; Fikrová, Petra (advisor) ; Kudláčková, Zděnka (referee)
Spikova Andrea Renal pathology and diagnostic methods Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Field of study: Medical Laboratory Technician This thesis is focused on diagnostic methods in various renal diseases. The first part is devoted to the anatomy and physiology of the kidney, where is outlined how look and operate various parts of kidneys and also the whole organ. Next part si composed of analysis of the urine, where is described chemical analysis with test strip, when the colore change of indicator of zones subtracted result according to the color scale on the container tube. Nowadays are two types of devices for reading the diagnostic strips on the market. Basic reflex photometer operated by person or fully automatic one. Further part describe the examination of urinary sediment using light microscopy or phase contrast microscopy. In modern biochemical laboratories urinary sediment is investigated using flow cytometry or digital capture particles. The last part is devoted to imaging methods and renal biopsy, to their description, indications and pa comparison with another method. Imaging methods include ultrasonography, native image renal, excretory urography, ascendent ureter/pyelography, computed tomography, multidetector computed tomography,...
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The effect of physical activity and nutrition interventions on physical fitness and quality of life during the first year after kidney transplantation
Švagrová, Klára ; Bunc, Václav (advisor) ; Brandejský, Petr (referee) ; Adámková, Věra (referee)
UNIVERZITA KARLOVA V PRAZE FAKULTA TĚLESNÉ VÝCHOVY A SPORTU The effect of physical activity and nutrition interventions on physical fitness and quality of life during the first year after kidney transplantation Abstract of disertation Zpracovala: Mgr. Klára Švagrová Školitel: Prof. Ing. Václav Bunc, CSc. Školící pracoviště: Laboratoř sportovní motoriky Praha, 2012 ABSTRACT Kidney transplantation is the best known treatment of chronic kidney failure. However, it is often accompanied by a number of health complications. The transplantation itself positively affects both physical fitness and quality of life during the first year after the transplant surgery which are seriously diminished when compared to the healthy population. This improvement can be even enhanced by appropriate physical activity and nutrition interventions otherwise both the physical fitness and quality of life would start declining after the first post-transplant year again. The aim of this study was to confirm a positive effect of a long-term physical activity and nutrition interventions on health-related physical fitness and health-related quality of life in a representative sample of individuals in the first year after the kidney transplantation. At the same time it was fundamental to verify that the experimental trial can be...
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Renal pathology and diagnostic methods
Špiková, Andrea ; Fikrová, Petra (advisor) ; Kudláčková, Zděnka (referee)
Spikova Andrea Renal pathology and diagnostic methods Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Field of study: Medical Laboratory Technician This thesis is focused on diagnostic methods in various renal diseases. The first part is devoted to the anatomy and physiology of the kidney, where is outlined how look and operate various parts of kidneys and also the whole organ. Next part si composed of analysis of the urine, where is described chemical analysis with test strip, when the colore change of indicator of zones subtracted result according to the color scale on the container tube. Nowadays are two types of devices for reading the diagnostic strips on the market. Basic reflex photometer operated by person or fully automatic one. Further part describe the examination of urinary sediment using light microscopy or phase contrast microscopy. In modern biochemical laboratories urinary sediment is investigated using flow cytometry or digital capture particles. The last part is devoted to imaging methods and renal biopsy, to their description, indications and pa comparison with another method. Imaging methods include ultrasonography, native image renal, excretory urography, ascendent ureter/pyelography, computed tomography, multidetector computed tomography,...
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The effect of physical activity and nutrition interventions on physical fitness and quality of life during the first year after kidney transplantation
Švagrová, Klára ; Bunc, Václav (advisor) ; Brandejský, Petr (referee) ; Adámková, Věra (referee)
UNIVERZITA KARLOVA V PRAZE FAKULTA TĚLESNÉ VÝCHOVY A SPORTU The effect of physical activity and nutrition interventions on physical fitness and quality of life during the first year after kidney transplantation Abstract of disertation Zpracovala: Mgr. Klára Švagrová Školitel: Prof. Ing. Václav Bunc, CSc. Školící pracoviště: Laboratoř sportovní motoriky Praha, 2012 ABSTRACT Kidney transplantation is the best known treatment of chronic kidney failure. However, it is often accompanied by a number of health complications. The transplantation itself positively affects both physical fitness and quality of life during the first year after the transplant surgery which are seriously diminished when compared to the healthy population. This improvement can be even enhanced by appropriate physical activity and nutrition interventions otherwise both the physical fitness and quality of life would start declining after the first post-transplant year again. The aim of this study was to confirm a positive effect of a long-term physical activity and nutrition interventions on health-related physical fitness and health-related quality of life in a representative sample of individuals in the first year after the kidney transplantation. At the same time it was fundamental to verify that the experimental trial can be...
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The role of IL-17 in kidney transplantation
Menšíková, Markéta ; Krulová, Magdaléna (referee) ; Stříž, Ilja (advisor)
The role of IL-17 in kidney transplantation - abstract Naive CD4+ T-lymphocytes (Thp) can develop into Th17 line in the presence of TGF- and IL-6. Th17 cells are characterized by expression of Ror- t and by production of interleukin-17 (IL-17). It is secreted as a glycoprotein homodimer. Binding to IL-17 receptor (IL-17R), which is present in all cell types, stimulates the production of proinflammatory cytokines and chemokines. The ratio of Th17: Treg in the graft showing signs of rejection is higher than in the graft without rejection. The presence of IL-17 in a culture of proximal tubular epithelial cells (PTEC) stimulates the production of IL-6, IL-8, MCP-1 and C3 complement component. Simultaneous action of IL-17 and CD40L synergistically increases the production of IL-6, IL-8 and RANTES. Signaling from the receptor on the surface of PTEC associated with its increased expression is effected via the src kinase and MAP kinase, and probably leads to the transcription factor NF- B. In rat models of transplantation, the IL-17 appears in allografts on the second day after surgery, the level rises until the fifth day, then decreases and disappears before the death of the animal. IL-17 is not detectable in isografts and negative controls. It appears before the IFN- , which had been considered a trigger of...
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