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National Repository of Grey Literature

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Voltammetric Analysis of Anthraquinone-labeled Nucleotide Triphosphates and Oligonucleotides at Gold Electrodes Vidláková, Pavlína ; Balintová, Jana ; Havran, Luděk ; Hocek, Michal ; Fojta, Miroslav DNA labelling is used for increase of sensitivity of electrochemical detection. When the DNA or oligonucleotides (ONs) are chemically modified, their electrochemical responses can be changed depending on electrochemical activity of the introduced moiety. One option of DNA labeling is incorporation of chemically modified nucleotides using corresponding deoxynucleotide triphosphates (dNTPs) into DNA molecules with using primer extension (PEX). In our study we used anthraquinone labeled dNTPs and ONs with enzymatically incorporated anthraquinone labeled nucleotides. Thus synthesized ON stretch bearing the anthraquinone tags was electrochemically analyzed using voltammetry at gold electrodes. Detailed record

Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode Ferenčíková, Z. ; Daňhel, Aleš ; Riedl, Jan ; Hocek, Michal ; Fojta, Miroslav 4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is electrochemically reducible and therefore it is possible to use alternatively the API-modified nuclotides as DNA redox labels. The electrochemical behavior of labeled nucleotides and labeled DNA was studied using cyclic voltammetry and transfer stripping cyclic voltammetry at hanging mercury drop electrode. Observed CVs showed irreversible reduction signals of 4-aminophthalimide label without production of consequently oxidizable/reducible species. Detailed record

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