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Extraction, separation and identification of anthocyans in skin of red cultivars of Vitis vinifera
Watzková, Jarmila ; Vespalcová, Milena (referee) ; Hrstka, Miroslav (advisor)
This diploma thesis deals with application of pressurized liquid extraction (PFE) on grape skins of Vitis vinifera cultivars Alibernet and Svatovavřinecké to gain extraction of anthocyanin pigments in the form of 3-monoglucosides. This thesis deals with comparison efficiency pressurized liquid extraction (PFE) for the range temperatures 40–120 °C and the Soxhlet extraction with four extraction solvents. The identification of the anthocyanin pigments by high-performance liquid chromatography with diode array detection (HPLC/DAD) based on Synergi C12 column separation (Phenomenex) was performed. The anthocyanin pigments were identified at wavelength 520 nm. The complete amount of anthocyanin pigments grape skins was depended on the extraction solvent and temperature in range 0,38–244,06 mg/g for cultivar Alibernet and 0,28–105,01 mg/g for cultivar Svatovavřinecké. The contents of anthocyanin pigments was determined in the samples of wine both cultivars in the range 3,83–2836,84 mg/l too. PFE followed by HPLC/DAD employing Synergi C12 column provides relatively fast, quantitative and reproducible determination of anthocyanins in grape skins and wines.
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Comparison of Extraction Methods for Isolation of 20-Hydroxyecdysone from Leuzea carthamoides
Machalová, Zdeňka ; Sajfrtová, Marie ; Sovová, Helena ; Bártlová, Milena
This work is focused on the extraction of 20-HE from roots and seeds of Leuzea carthamoides using SFE, PLE and Soxhlet extraction. The SFE and PLE conditions (pressure, temperature, type and concentration of solvent, extraction time) were optimized to improve extraction yields and concentrations of 20-HE in extracts. These methods were compared with a conventional Soxhlet-type extraction. The SFE experiments were carried out at temperatures from 40°C to 60°C, pressures from 30 to 65 MPa and with addition of 5-20% of co-solvent (ethanol, methanol and acetone). PLE with ethanol, methanol and acetone was made at temperatures from 70°C to 100°C, pressures from 7 to 20 MPa and extraction time from 5-15 min. Soxhlet extraction with ethanol was used to evaluate the total content of 20-HE in roots and seeds. The concentration of 20-HE in extracts was determined by HPLC.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22014082908303 - PDF
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