|
|
Oxidative and carbonyl stress in kidney diseases
Kratochvílová, Markéta ; Tesař, Vladimír (advisor) ; Průša, Richard (referee) ; Zadražil, Josef (referee)
Aims: 1. Determination of AGEs (Advanced Glycation End products) in patients with various types of nephropathy. 2. Association AGEs with nutritional parameters and anemia. 3. Influence of renal parameters on sRAGE (soluble form of Receptor for Advanced Glycation End products) levels. 4. Technics and proceeding methods of the podocytes cultivation. 5. Determination of urine podocytes. Methods: We determined fluorescent AGEs by spectrofluorometry, sRAGE by Enzyme-Linked ImmunoSorbent Assay (ELISA). Podocytes were passaged and identified immunocytochemically. Podocytes in urine were specified by flow cytometry method. Results: 1. We did not find significant differences in AGEs serum levels among various types of nephropathy, even though the pathogenesis differs. 2. The albumin and prealbumin levels positively and haemoglobin levels negatively correlate with AGEs in patients with CKD grade 1-5, without necessity of dialysis. 3. Serum sRAGE levels are increased in patients with decreased renal function independently on the course of renal disease. 4. We implemented the methods and technics of podocyte cultivation. 5. Urine podocytes observation and confirmation that podocyturia relates to disease activity. Conclusion: We confirmed that AGEs serum levels depend more on renal function than the type of...
|
|
|
The effect of high protein enteral nutrition on the nutritional status of the patients in intesive care
Jirků, Jitka ; Křížová, Jarmila (advisor) ; Zlatohlávek, Lukáš (referee)
Background: The paper presents a broad view of the issue of providing sufficient nutritional support (enteral nutrition) with a high level of protein for the target group of patients in intensive care. Patients in these types of departments (ARO, ICU) have different metabolic requirements at different time intervals of their acute illness. Understanding the essence of metabolism of essential macronutrients with all the consequences on the organism is the basis for understanding metabolism in intensive care with a significant influence on the different nutritional requirements of these patients and maximizing our efforts to adapt the type of enteral nutrition to the needs of intensive care patients. Objective: The aim of this work was to evaluate the effect of the new enteral formula Peptamen Intense on the nutritional status of patients in intensive care. Assess the possibility of achieving the nutritional target of proteins (energy) according to the guidelines. Evaluate other parameters that influence management of nutritional support in patients with intensive care. Methodology: Ten patients were selected from the workplaces (ARO, JIP) throughout the Czech Republic according to clearly defined input criteria between November 2017 and March 2018. Subsequently, all patients were enrolled in the...
|
|
|
Oxidative and carbonyl stress in kidney diseases
Kratochvílová, Markéta ; Tesař, Vladimír (advisor) ; Průša, Richard (referee) ; Zadražil, Josef (referee)
Aims: 1. Determination of AGEs (Advanced Glycation End products) in patients with various types of nephropathy. 2. Association AGEs with nutritional parameters and anemia. 3. Influence of renal parameters on sRAGE (soluble form of Receptor for Advanced Glycation End products) levels. 4. Technics and proceeding methods of the podocytes cultivation. 5. Determination of urine podocytes. Methods: We determined fluorescent AGEs by spectrofluorometry, sRAGE by Enzyme-Linked ImmunoSorbent Assay (ELISA). Podocytes were passaged and identified immunocytochemically. Podocytes in urine were specified by flow cytometry method. Results: 1. We did not find significant differences in AGEs serum levels among various types of nephropathy, even though the pathogenesis differs. 2. The albumin and prealbumin levels positively and haemoglobin levels negatively correlate with AGEs in patients with CKD grade 1-5, without necessity of dialysis. 3. Serum sRAGE levels are increased in patients with decreased renal function independently on the course of renal disease. 4. We implemented the methods and technics of podocyte cultivation. 5. Urine podocytes observation and confirmation that podocyturia relates to disease activity. Conclusion: We confirmed that AGEs serum levels depend more on renal function than the type of...
|
guest ::
