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The impact of intracytoplasmic sperm injection on succesful development and fertility in selected mammal species
Kaufman, Jonáš ; Petelák, Aleš (advisor) ; Petr, Jaroslav (referee)
5 Abstract This thesis aims to summarize the current knowledge about the development and reproductive capacities of individuals conceived by artificial conception, especially intracytoplasmic sperm injection (ICSI), and to make a comparison with naturally conceived ones. The focus is primarily on human assisted reproduction, even though scientific work with human gametes and embryos faces ethical difficulties. However, the main objective is to determine whether ICSI has a negative effect on offspring, partially using results from animal models. Furthermore, this thesis compares birth outcomes, incidence of birth defects, cognitive and reproductive abilities and both genetic and epigenetic integrity of individuals conceived naturally with those conceived via ICSI. As of yet, according to available information, ICSI appears to be a safe artificial method of reproduction. Poor results may often either be explained by the reduced fertility of parents, or their significance is negligible. Keywords: artificial fertilization, ART, ICSI, reproduction, offspring, mouse, livestock, human
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