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Development of Gel Techniques for in situ Measurement of Bioavailable Metals in Soils and Sediments
Kovaříková, Vladěna ; Sommer, Lumír (referee) ; Janoš, Pavel (referee) ; Dočekalová, Hana (advisor)
The doctoral thesis deals with usage of gel techniques for in situ measurement of bioavailable metals in soils and sediments. The DGT technique was applied to soil samples from the localities of Zlín, Tuřany and Chrlice for estimation of bioavailable metals in soils. These soils were amended by sewage sludges in years 1980-1987. The experiment using diffusive gels with different pore size, APA and RG gels, has shown that the DGT can be used for assessment of trace metal soil complexes - small inorganic and larger organic. The results obtained by DGT measurements were approximately up to two orders of magnitude lower than metal concentrations obtained by leaching with sodium nitrate. The leaching with sodium nitrate does not provide true reflection of metal availability to plant root system and soil microorganisms and the DGT technique seems to be a better predictor of metal bioavailability. Only small differences between undisturbed and homogenized soil samples were found out by application of DGT to soil samples from localities of Veverská Bítýška and Ostrovačice. The undisturbed soil is not homogenous and the DGT unit gives information only about the part of the soil which is in direct contact with the unit. The procedure with soil slurry can be recommended for the characterization of large soil areas and estimation of bioavailable metals in soils. Gel techniques were also used for the study of processes occuring in sediments. Mercury species and other metals were analysed in surface water, pore water and sediments collected from two sampling sites in the Dele River in northern France. This river had been polluted during the last century by the activity of Métaleurop factory, which dealt with metal ore treatment. The application of DGT technique using two specific ion-exchange resins, Chelex-100 and Duolite GT-73, provided assessment of mercury in sediment pore water. DGT and DET techniques together with centrifugation provided also high resolution depth profiles of Fe, Mn, Zn, Pb, Cr and Cd in pore water. It is not possible to determine which sampling site was more contaminated. Only small differences in mercury species and metal content were observed between sampling sites. The Dele River can be classified as contaminated river according to total mercury content in sediments and as slightly contaminated river from the point of view of methylmercury content. Correlations between mercury species and sulphide content confirm that total mercury and sulphide content count among factors influencing the mercury release to pore water and controlling processes of methylation and demethylation. Only low toxicity of sediment slurries was found out by the bioluminiscence test with Vibrio fischeri. The results of doctoral thesis demonstrate gel techniques as a very good tool in environmental analysis.
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Growth of aquatic vegetation in fish ponds used for recreation - Bolevecká soustava near Plzeň.
Pták, Martin ; Duras, Jindřich (advisor) ; Potužák, Jan (referee)
The aim of this diploma thesis was to clarify the differences among three different ponds (Bolevecký, Třemošenský, Šídlovský) situated in Pilsen. All of the above mentioned ponds are recreational areas where Bolevecký pond is best known for his water quality improvements since 2006 (after application of Fe and Al - colagulants and also fish - stock changes). The main difference was based on the growth of submersed water plants. The macrophytes were growing slowly or not at all in Bolevecký pond. There was a huge expansion of macrophytes (mainly Myriophyllum spicatum species) in Třemošenský and Šídlovský ponds therefore the water plants must be regulated during summer seasons by mechanical harvesting. The water in all three ponds proves the same qualities but the differences may be found in the nutrient composition of sediments. The fractionation analysis (the founders Psenner and Puczsko) confirmed the fact that all localities are poor in phosphorus however the differences were found in content of iron. The increased content of iron was observed in sediments of Šídlovksý and Třemošenský ponds (mainly in BD fraction - the iron was unstable under redox potential changes). The result of this analysis proves that phosphorus is potentially more available for macrophytes in sediments of Třemošenský and Šídlovský...
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Development of Gel Techniques for in situ Measurement of Bioavailable Metals in Soils and Sediments
Kovaříková, Vladěna ; Sommer, Lumír (referee) ; Janoš, Pavel (referee) ; Dočekalová, Hana (advisor)
The doctoral thesis deals with usage of gel techniques for in situ measurement of bioavailable metals in soils and sediments. The DGT technique was applied to soil samples from the localities of Zlín, Tuřany and Chrlice for estimation of bioavailable metals in soils. These soils were amended by sewage sludges in years 1980-1987. The experiment using diffusive gels with different pore size, APA and RG gels, has shown that the DGT can be used for assessment of trace metal soil complexes - small inorganic and larger organic. The results obtained by DGT measurements were approximately up to two orders of magnitude lower than metal concentrations obtained by leaching with sodium nitrate. The leaching with sodium nitrate does not provide true reflection of metal availability to plant root system and soil microorganisms and the DGT technique seems to be a better predictor of metal bioavailability. Only small differences between undisturbed and homogenized soil samples were found out by application of DGT to soil samples from localities of Veverská Bítýška and Ostrovačice. The undisturbed soil is not homogenous and the DGT unit gives information only about the part of the soil which is in direct contact with the unit. The procedure with soil slurry can be recommended for the characterization of large soil areas and estimation of bioavailable metals in soils. Gel techniques were also used for the study of processes occuring in sediments. Mercury species and other metals were analysed in surface water, pore water and sediments collected from two sampling sites in the Dele River in northern France. This river had been polluted during the last century by the activity of Métaleurop factory, which dealt with metal ore treatment. The application of DGT technique using two specific ion-exchange resins, Chelex-100 and Duolite GT-73, provided assessment of mercury in sediment pore water. DGT and DET techniques together with centrifugation provided also high resolution depth profiles of Fe, Mn, Zn, Pb, Cr and Cd in pore water. It is not possible to determine which sampling site was more contaminated. Only small differences in mercury species and metal content were observed between sampling sites. The Dele River can be classified as contaminated river according to total mercury content in sediments and as slightly contaminated river from the point of view of methylmercury content. Correlations between mercury species and sulphide content confirm that total mercury and sulphide content count among factors influencing the mercury release to pore water and controlling processes of methylation and demethylation. Only low toxicity of sediment slurries was found out by the bioluminiscence test with Vibrio fischeri. The results of doctoral thesis demonstrate gel techniques as a very good tool in environmental analysis.
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Study of properties DET, a method for pore water for sediment sampling
JAROLÍMOVÁ, Zuzana
The aim of this thesis was find out chosen properties of DET (diffusive equilibration in thin films), method for pore water for sediment sampling. This properties were: 1)the time require for equilibration between: a)distilled water in gel within DET and pore water b)sample of pore water within DET and distilled water 2)the comparison of samples pore water sampling with DET and other method 3)determination of DET properties in practice
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Changes of phosphorus and iron concentrations in pore water vertical profiles of hypertrophic reservoir measured by gel minipeepers.
PECHÁČKOVÁ, Petra
Sediment cores obtained during the year 2006 (April, June, September), originated from inflow and dam sites in hypertrophic reservoir were incubated and analyzed in the laboratory under in situ conditions. The aim of this study was to find out the release rates of phosphorus and iron and to define the changes in the concentrations of dissolved compounds in pore water in the first 6 cm in the sediment. Gel minipeepers were used to measure the vertical profiles of dissolved analyts in pore water. This method was found suitable for investigation of P, Fe and basic ions concentrations. Differences between inflow and dam site were found
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