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Determination of active substances and possible degradation products in a historical sample of Algena from 1981
Bauerová, Markéta ; Nesměrák, Karel (advisor) ; Kozlík, Petr (referee)
The Algena tablet was an over-the-counter drug in the second half of the twentieth century in Czechoslovakia. Algena was mainly used against pain. The tablet contains four active substances: aminophenazone, aprobarbital, phenacetin, and caffeine. The aim of this thesis was to analyse the active substances in the Algena tablet from 1981,determinate theircontent,and possible degradation products.To complete these goals the RP-HPLC method with UV detection had to be involved and optimized.HPLC-MS was used to detect possible degradation products. Separation was performed on the XBridge® BEH C18 column (150×3.0 mm; 2.5 µm), binary elution was used. The first component of the mobile phase was0.1% aqueous solution of acetic acid and the second component was methanol. The determined contents of active substances (decelerated by the manufacturer) were: amino- phenazone 97.0%,aprobarbital89.2%, phenacetin 96.0% and caffeine 93.2%.Degradation products of the active substances were not detected by HPLC with MS detection. Key words: Algena, aminophenazone, aprobarbital, caffeine, degradation, phenacetin, RP-HPLC
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