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Programmable illuminating system for an optical microscope
Lošťák, Martin ; Křupka, Ivan (referee) ; Chmelík, Radim (advisor)
A programmable illuminating system (PIS) uses a commercial multimedia projector together with a suitable optical relay system in order to illuminate specimens under microscope with transmitted light. The theoretical part of the diploma thesis describes some methods used in the optical transmission microscopy. All of these methods employ physical masks placed in the condenser front focal plane. In the case of the traditional methods the masks are used to enhance contrast (e.g. dark-field illumination) and resolution (oblique illumination). One of the methods (a condenser with rotating aperture) provides the information about the three-dimensionality of the specimen. The next part of the thesis contains the theory and the basic classification of the illuminating systems used in the optical transmission microscopy. An optical and mechanical design of the optical relay system used for PIS is introduced. The experimental part shows the results made with two different PIS arrangements. It was shown on two different specimens that the PIS provides the same illumination as the classical methods. It was also proved that the PIS can simulate the rotating aperture in the condenser front focal plane and thus to give the information about the three-dimensionality of the specimen. Some new static and dynamic illuminating methods were introduced.
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Study of the behavior of selected rocks at high temperatures
Holanec, Aleš ; Sitek,, Libor (referee) ; Bodnárová, Lenka (advisor)
The diploma thesis deals with the observation and diagnostics of transformations that take place in stone slabs of different mineralogical composition during heating to high temperatures. The theoretical part of the thesis deals with a summary of available information about selected types of rocks. Furthermore, it presents findings from the experimental thermal loading of a granite sample. The principles and instruments of laboratory experimental methods that are used to judge the resistance of rocks are described in detail. In the experimental part, the testing methodology is proposed, the procedure of production of rock test specimens is described and the way in which the thermal loading of stone slabs took place is explained. The results of the experiments are shown in the tables and graphs. A comparison of individual tests and properties of selected samples and evaluation of the obtained results is performed.
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Probiotics and prebiotics - a study of interactions, effects and co-encapsulation
Vrtná, Monika ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
This diploma thesis is focused on encapsulation probiotics and co-encapsulation with some types of prebiotics. In theoretical part is aimed to probiotics, their general characteristics and application of probiotics in food industry. There are described prebiotics and their classification, there is described principles of encapsulation and encapsulation techniques. Methods, which are used for analysis of particles and encapsulation components were introduced too. The experimental part describes methods of prebiotics characterization by high performance liquid chromatography, thin layer chromatography and spectrophotometric methods. Cultivation of probiotics with prebiotics - hydrolyzed and non-hydrolyzed wad tested. Using flow cytometry cell viability was measured too. Finally probiotics and prebiotics were encapsulated, mainly by encapsulator machine. Long-term stability of particles during 6 week storage was observed. The particles were exposed to effect of artificial intestinal, gastric and bile juices.
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Design of Microscope LED Illumination Source
Bartoň, Luboš ; Hartl, Martin (referee) ; Šperka, Petr (advisor)
This master’s thesis deals with the construction design of microscope episcopic LED illumination source and subsequent functional sample realization. Illumination source is compatible with industrial microscope Nikon Eclipse LV150 and designed as a replacement for halogen light source. Way of collecting light is chosen with respect to search analysis results and collector optical element and LED is selected with respect to the analysis of the episcopic illuminator. Two construction designs are designed with respect to the results of photometric analyzes. Optimal design is realized and compared with used lighting sources – halogen and xenon.
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Encapsulation of lactic acid bacteria
Vrtná, Monika ; Lichnová, Andrea (referee) ; Márová, Ivana (advisor)
This bachelor thesis is focused on encapsulation of two strains of probiotic bacteria. Lactic acid bacteria were encapsulated into several types of materials, mostly polysaccharides. The theoretical part describes the techniques of encapsulation applicable in the food industry. During the experimental part alginate and chitosan microparticles were prepared. These particles exhibited different sizes and some of them were modified by starch and carboxymethyl cellulose. Particles were used for encapsulation of two strains - Bifidobacterium breve and Lactobacillus acidophilus. Using flow cytometry cell viability was measured after 24-hours cultivation of bacteria. Particles were observed immediately after encapsulation by optical microscopy and then long-term stability in model foods was evaluated after two days, a week and after four weeks of incubation. Using the Bürker chamber the alived and death bacteria were counted inside and outside the capsule. The stability and viability of the cells were studied also in the artificial intestinal, stomach and bile juices. As the best material for encapsulation of lactic acid bacteria 2% alginate modified by carboxymethyl cellulose was found. The highest viability of bacteria was observed in milk as the real model food. Prepared particles are suitable for use in the food industry.
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Condition of dorsoabdominal scent glands in adults of the true bugs from the taxon Pentatomomorpha (Heteroptera)
Křížková, Petra ; Vilímová, Jitka (advisor) ; Kment, Petr (referee)
Existence of larval dorsoabdominal scent glands (DAGs) together with occurence of adult metathoracic scent glands is one of the important autapomorphic characters of taxon Heteroptera within taxon Insecta. DAGs can persist until adults in some species of this taxon. The persistence of DAGs were proved also in members of infraorder Pentatomomorpha. Targets of this thesis are: collect the knowledge about persistent adult DAGs in selected representative members of important families of taxon Pentatomomorpha; study and compare the condition of the DAGs in the oldest larval instars and adults; clarify the significance of existence the openings of conducting ductules of proper glandular units in reservoir intima of DAGs in study of the persistence of these glands. For study were used stereomicroscope, light and scanning electron microscope. Key words: Pentatomomorpha, Heteroptera, dorsoabdominal scent glands, openings of conducting ductules of glandular units, larvae of 5th instar, persistence in adults, persistence patterns, light microscope, scanning electron microscope
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Study of the behavior of selected rocks at high temperatures
Holanec, Aleš ; Sitek,, Libor (referee) ; Bodnárová, Lenka (advisor)
The diploma thesis deals with the observation and diagnostics of transformations that take place in stone slabs of different mineralogical composition during heating to high temperatures. The theoretical part of the thesis deals with a summary of available information about selected types of rocks. Furthermore, it presents findings from the experimental thermal loading of a granite sample. The principles and instruments of laboratory experimental methods that are used to judge the resistance of rocks are described in detail. In the experimental part, the testing methodology is proposed, the procedure of production of rock test specimens is described and the way in which the thermal loading of stone slabs took place is explained. The results of the experiments are shown in the tables and graphs. A comparison of individual tests and properties of selected samples and evaluation of the obtained results is performed.
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