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Finding guanine quadruplexes in DNA using decision trees
Kotrys, Kryštof ; Šťastný, Jiří (referee) ; Kaura, Patrik (advisor)
This bachelor’s thesis is focused on creating a functional decision tree model for detection of guanine quadruplexes in the DNA. The first part of the thesis summarizes theoretical knowledge in the fields of local DNA structures, computational prediction methods of guanine quadruplexes and decision trees. The second part describes the creation of a decision tree model, followed by a statistical comparison of the results with the G4Hunter algorithm.
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Finding guanine quadruplexes in DNA using decision trees
Kotrys, Kryštof ; Šťastný, Jiří (referee) ; Kaura, Patrik (advisor)
This bachelor’s thesis is focused on creating a functional decision tree model for detection of guanine quadruplexes in the DNA. The first part of the thesis summarizes theoretical knowledge in the fields of local DNA structures, computational prediction methods of guanine quadruplexes and decision trees. The second part describes the creation of a decision tree model, followed by a statistical comparison of the results with the G4Hunter algorithm.
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New C-H activations and cross-coupling reactions for modification of deazapurine nucleobases
Sabat, Nazarii ; Hocek, Michal (advisor) ; Tobrman, Tomáš (referee) ; Hlaváč, Jan (referee)
This PhD thesis reports the development of novel C-H activation strategies and aqueous-phase Suzuki-Miyaura cross-coupling reactions for the synthesis of modified deazapurine nucleobases. The methodologies of chemo- and regioselective synthesis of highly functionalized deazapurines have been developed by using modern C-H activation chemistry. Various functional groups such as amino-, imido-, silyl- and phosphonyl- were introduced by C-H activation reactions. Amino deazapurine derivatives were synthesized by developed Pd/Cu-catalyzed direct C-H amination and C-H chloroamination of 6-substituted 7-deazapurines with N-chloro-N- alkyl-arylsulfonamides. C-H imidation reactions of pyrrolopirimidines were performed under ferrocene catalysis with N-succinimido- or N-phtalimidoperesters. In order to obtain silylated derivatives, Ir-catalyzed C-H silylations of phenyldeazapurines with alkyl silanes were designed. Highly interesting deazapurine phosphonates were prepared by using Mn-promoted C-H phosphonation method and were further transformed into the corresponding phosphonic acids. All of the developed direct C-H functionalization reactions proceeded regioselectively at position 8 in deazapurine core, except for C-H silylation where reaction undergoes mainly as directed ortho C-H silylation on phenyl ring,...
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