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Preparation and Characterization of Drug Carrier Systems on the Basis of Magnetic Nanoparticles
Chvátalová, Barbora ; Burdíková, Jana (referee) ; Kalina, Michal (advisor)
This bachelor thesis deals with the study of magnetic nanoparticles, optimizing their preparation and stabilization. The nanoparticles with different ratios of Fe2+ and Fe3+ ions were prepared by coprecipitation by variously concentrated sodium hydroxide. Three samples of particles were selected and were further observed. Their stability in Milli-Q water, buffer, polyvinylalcohol, hyaluronic acid and chitosan was studied in function of time using methods of dynamic and electrophoretic light scattering. By UV-VIS spectrometry it was confirmed that the magnetic nanoparticles were actually prepared. Nanoparticles coated with chitosan and hyaluronic acid were then further characterized using FT-IR, XRD and SEM.
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Hyaluronan-Micelle Aggregates and their Potential for Nanomedicine Applications
Pilgrová, Tereza ; Burgert, Ladislav (referee) ; Reháková, Milena (referee) ; Pekař, Miloslav (advisor)
This thesis deals with the study of preparation and characterization of hyaluronan-micelle aggregates. The theoretical part deals with drug delivery systems, characterization of used materials and methods especially fluorescence spectroscopy, dynamic light scattering and turbidimetry. Methods of determination of measured data are summarized in the experimental section. The result section is divided into two subsections dedicated to different preparation methods of hyaluronan-surfactant complexes. Induced aggregates of hyaluronan with Septonex are characterized in terms of their origin and stability, and the results are compared with previously studied surfactants CTAB. In the second part are discussed so-called decorated micelles, their formation, properties and stability.
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Tailoring transport properties of controlled release systems based on polyhydroxyalkanoates.
Apsalikova, Zarina ; Obruča, Stanislav (referee) ; Sedláček, Petr (advisor)
V rámci předložené práci, polyhydroxyalkanoáty s odlišnými monomerními jednotkami, jako 3-hydroxyvalerát, 4-hydroxyvalerát, 4-hydroxyhexanoát, a 5-hydroxyvalerát byly připravovány za využití bakteriálního kmenu Aneurinibacillus (AH30, AFN2) za účelem porovnání transportních vlastnosti nosičů na bázi izolovaných kopolymerů. Na základě výsledku plynové chromatografii (GC-FID) se ukázalo, izoláty jsou schopné produkovat PHA s různým procentuálním zastoupením monomerů. Dále molekulová hmotnost a index polydispezity izolovaných kopolymerů byly analyzovány pomoci SEC-MALS. Samotné nosičové systémy byly připravovány s využitím izolovaných kopolymerů a komerčního poly(3-hydroxyburyrát). Optimizace byla zaměřena na nastavení podmínek pro tvorbu částic, filmu a porézního systému. Zkoumanými podmínkami byly koncentrace roztoku, množství pomocné látky, a proces izolace nosičů. Vytvořené nosičové systémy byly charakterizovány z pohledu jejich schopnosti uvolňovat aktivní látku (ibuprofen) do modelového prostředí (fosfátový pufr). Na základě provedené charakterizaci bylo stanoveno, že transportní vlastnosti jsou ovlivněny strukturou polymeru a interakcemi v systému polymer-rozpouštědlo. Skenovací elektronová mikroskopie, diferenciální skenovací kalorimetrie, infračervená spektroskopie s Fourierovou transformaci a UV-VIS spektroskopie byly využity jako nástroje pro porovnání a pochopení korespondence mezi chemickou strukturou PHA a jejich transportními vlastnostmi.
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Hyaluronan-Micelle Aggregates and their Potential for Nanomedicine Applications
Pilgrová, Tereza ; Burgert, Ladislav (referee) ; Reháková, Milena (referee) ; Pekař, Miloslav (advisor)
This thesis deals with the study of preparation and characterization of hyaluronan-micelle aggregates. The theoretical part deals with drug delivery systems, characterization of used materials and methods especially fluorescence spectroscopy, dynamic light scattering and turbidimetry. Methods of determination of measured data are summarized in the experimental section. The result section is divided into two subsections dedicated to different preparation methods of hyaluronan-surfactant complexes. Induced aggregates of hyaluronan with Septonex are characterized in terms of their origin and stability, and the results are compared with previously studied surfactants CTAB. In the second part are discussed so-called decorated micelles, their formation, properties and stability.
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Preparation and Characterization of Drug Carrier Systems on the Basis of Magnetic Nanoparticles
Chvátalová, Barbora ; Burdíková, Jana (referee) ; Kalina, Michal (advisor)
This bachelor thesis deals with the study of magnetic nanoparticles, optimizing their preparation and stabilization. The nanoparticles with different ratios of Fe2+ and Fe3+ ions were prepared by coprecipitation by variously concentrated sodium hydroxide. Three samples of particles were selected and were further observed. Their stability in Milli-Q water, buffer, polyvinylalcohol, hyaluronic acid and chitosan was studied in function of time using methods of dynamic and electrophoretic light scattering. By UV-VIS spectrometry it was confirmed that the magnetic nanoparticles were actually prepared. Nanoparticles coated with chitosan and hyaluronic acid were then further characterized using FT-IR, XRD and SEM.
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