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The use of milling and co-milling procedures for preparation of interactive mixtures
Jezerská, Jana ; Šklubalová, Zdeňka (advisor) ; Kvítek, Libor (referee) ; Vitková, Zuzana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Candidate: Mgr. Jana Jezerská Supervisor: doc. PharmDr. Zdeňka Šklubalová, Ph.D. Consultant: Ing. Jakub Koktan, Ph.D. Title of Doctoral Thesis: Utilization of technological procedures of milling and co-milling in the preparation of interactive mixtures The dissertation thesis is an annotated summary of the publication and research activities of the author, Mgr. Jana Jezerská (maiden name Brokešová). The thesis is focused on the preparation of binary interactive powder mixtures which consist of the micronized drug particles adhered onto the larger particles of an excipient (a carrier). The binary powder mixtures were prepared by mixing and/or co-milling and characterized by granulometric methods; crystallinity (thermal analysis, X-ray powder diffractometry), the flow properties (shear cell, avalanche properties) and the surface energy were evaluated as well. The dissolution rate of model drugs was estimated using a flow-through powder dissolution cell (USP-4). The used statistical model (central composite design) enabled to establish optimal milling conditions in a ball mill (the milling speed, the milling time, the size of milling balls) for five powder carriers. Based on quadratic response surface,...
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The use of milling and co-milling procedures for preparation of interactive mixtures
Jezerská, Jana ; Šklubalová, Zdeňka (advisor) ; Kvítek, Libor (referee) ; Vitková, Zuzana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Candidate: Mgr. Jana Jezerská Supervisor: doc. PharmDr. Zdeňka Šklubalová, Ph.D. Consultant: Ing. Jakub Koktan, Ph.D. Title of Doctoral Thesis: Utilization of technological procedures of milling and co-milling in the preparation of interactive mixtures The dissertation thesis is an annotated summary of the publication and research activities of the author, Mgr. Jana Jezerská (maiden name Brokešová). The thesis is focused on the preparation of binary interactive powder mixtures which consist of the micronized drug particles adhered onto the larger particles of an excipient (a carrier). The binary powder mixtures were prepared by mixing and/or co-milling and characterized by granulometric methods; crystallinity (thermal analysis, X-ray powder diffractometry), the flow properties (shear cell, avalanche properties) and the surface energy were evaluated as well. The dissolution rate of model drugs was estimated using a flow-through powder dissolution cell (USP-4). The used statistical model (central composite design) enabled to establish optimal milling conditions in a ball mill (the milling speed, the milling time, the size of milling balls) for five powder carriers. Based on quadratic response surface,...
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Possibilities of using stem cells for treatment of ocular surface injuries
Kössl, Jan ; Holáň, Vladimír (advisor) ; Drbal, Karel (referee)
Ocular surface defects are one of the most common causes of impaired vision or even blindness. Corneal transplantation is the first choice of the treatment of these defects. If the damage is extensive and includes the limbus, niche of limbal stem cells (LSCs), LSC deficiency (LSCD) occurs and reparation with regeneration of cornea is impaired. The only way to treat LSCD is limbal transplantation or transplantation of autologous LSCs from the healthy eye. In cases of bilateral LSCD there are no autologous LSCs available. The use of allogeneic LSCs is associated with the requirement of systemic or local administration of immunosuppressive drugs which have often negative side-effects and outcomes remain uncertain. The alternative for treatment of ocular surface defects and LSCD is finding a new source of an appropriate autologous stem cell substitute which are e.g. mesenchymal stem cells (MSCs). These cells can be obtained from bone marrow or adipose tissue of the particular patient. MSCs can be easily cultivated ex vivo and can be transferred onto the damaged ocular surface using appropriate scaffold. Here they can differentiate to corneal epithelial cells, have immunomodulatory properties and produce numerous trophic and growth factors. The experiments with MSCs on animal models of mechanically or...
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