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Noninvasive measurement of steroid homones and effect of hormonal manipulation on behaviour in the gecko Paroedura picta
Matušková, Lucie ; Kratochvíl, Lukáš (advisor) ; Fraňková, Marcela (referee)
Hormones influence life of all animals. Not only they affect physiological changes in organisms, but also impact their behaviour. This work focuses at two main groups of steroid hormones: glucocorticoids and androgens. Glucocortiods are activated in response to stress. Their levels can be measured using non-invasive methods, which have a range of advantages. The main advantage is the feedback-free sample collection for enzyme immunoassay. As the measurement involves metabolites of the hormones rather than the hormones themselves, prior validation of the method is, however, necessary. This work reports on a study aiming to validate non-invasive measurement on the Madagascar Ground Gecko (Paroedura Picta). The validation was based on ACTH challenge test: Synacthen Depot was injected, which should lead to increased blood level of glucocorticoids. The validation, however, was not successful. The measurement did not discover significant increase in the levels of the metabolites of glucocorticoids. In addition, the work focuses on behavioural effects of testosterone, the primary androgen. Hormonal manipulations have been carried out on several male and female specimens. The results have discovered differences in sexual behaviour between control groups. On the other hand, the hormonal manipulations had no...
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Noninvasive measurement of steroid homones and effect of hormonal manipulation on behaviour in the gecko Paroedura picta
Matušková, Lucie ; Kratochvíl, Lukáš (advisor) ; Fraňková, Marcela (referee)
Hormones influence life of all animals. Not only they affect physiological changes in organisms, but also impact their behaviour. This work focuses at two main groups of steroid hormones: glucocorticoids and androgens. Glucocortiods are activated in response to stress. Their levels can be measured using non-invasive methods, which have a range of advantages. The main advantage is the feedback-free sample collection for enzyme immunoassay. As the measurement involves metabolites of the hormones rather than the hormones themselves, prior validation of the method is, however, necessary. This work reports on a study aiming to validate non-invasive measurement on the Madagascar Ground Gecko (Paroedura Picta). The validation was based on ACTH challenge test: Synacthen Depot was injected, which should lead to increased blood level of glucocorticoids. The validation, however, was not successful. The measurement did not discover significant increase in the levels of the metabolites of glucocorticoids. In addition, the work focuses on behavioural effects of testosterone, the primary androgen. Hormonal manipulations have been carried out on several male and female specimens. The results have discovered differences in sexual behaviour between control groups. On the other hand, the hormonal manipulations had no...
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3D scanning technology in the hands of the conservator-restorer
Gláser, Petr
3D scanning technology is undoubtedly a modern progressive techniques that, especially in some areas, are increasingly applied. It is no different in the field of care for our cultural heritage. Relatively simple method brings, thanks to the quick development of means of implementation, still new applications and can deliver a wide range of important information. The devices are user friendly and comparatively affordable. These are just additional reasons for the fact that 3D scanning more applied in the fields of restoration. \nA short paper describes the technique of 3D scanning from the conservator-restorer's perspective and is focused primarily on understanding their capabilities, but does not forget those restrictions, which are always crucial. From time to time there is a tendency to uncritical promotion of certain methods or means. Many similar cases we also known in the field of cultural heritage and it seems that the 3D scanning method is well suited to become the next one.\n
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